Mezzetti G, Bagnara G, Monti M G, Bonsi L, Brunelli M A, Barbiroli B
Life Sci. 1984 May 28;34(22):2185-91. doi: 10.1016/0024-3205(84)90319-9.
Human histiocytic lymphoma cells (U-937) contain high affinity binding protein for 1 alpha, 25-dihydroxycholecalciferol. The Kd (0.2 nM) and sedimentation coefficient on sucrose gradients (3.7 S) are similar to those reported for 1 alpha, 25-dihydroxycholecalciferol receptor in other tissues. This secosteroid added to the culture was able to inhibit cell proliferation in a dose dependent manner. Differentiation-associated properties such as phagocytic ability, C3 rosette formation, positive reaction for alpha- naphtyl acetate esterase as well as electron microscopy examination indicate that 1 alpha, 25-dihydroxycholecalciferol induces in vitro monocyte-macrophage differentiation in this monoblast-like cell line.
人组织细胞淋巴瘤细胞（U - 937）含有1α,25 - 二羟胆钙化醇的高亲和力结合蛋白。其解离常数（Kd，0.2 nM）和在蔗糖梯度中的沉降系数（3.7 S）与其他组织中报道的1α,25 - 二羟胆钙化醇受体相似。添加到培养物中的这种甾体能够以剂量依赖的方式抑制细胞增殖。与分化相关的特性，如吞噬能力、C3玫瑰花结形成、α - 萘乙酸酯酶阳性反应以及电子显微镜检查表明，1α,25 - 二羟胆钙化醇在这种单核母细胞样细胞系中诱导体外单核细胞 - 巨噬细胞分化。
