表达病毒pp60src的BHK细胞中的酪氨酰和环磷酸腺苷依赖性蛋白激酶活性。
Tyrosyl and cyclic AMP-dependent protein kinase activities in BHK cells that express viral pp60src.
作者信息
Clinton G M, Roskoski R
出版信息
Mol Cell Biol. 1984 May;4(5):973-7. doi: 10.1128/mcb.4.5.973-977.1984.
Abstract
Protein kinase activities were measured in Rous sarcoma virus-infected baby hamster kidney (BHK) cells that express v-src (BHK [v-src]) and compared with those of revertant and control BHK cells. We observed about a fivefold-higher tyrosine phosphorylating activity in BHK (v-src) cell extracts, which was due to src but not other cellular tyrosyl kinase activities since preincubation with anti-src serum reduced the activity to control cell levels. The cyclic AMP-dependent protein kinase activity was also altered when v-src was expressed. Resolution of the two cyclic AMP-dependent isozymes from the detergent-soluble fraction of cells revealed that the type I activity was selectively decreased about fivefold in BHK (v-src) cells.
摘要
在表达v-src的劳氏肉瘤病毒感染的幼仓鼠肾（BHK）细胞（BHK [v-src]）中测量蛋白激酶活性，并与回复体和对照BHK细胞的蛋白激酶活性进行比较。我们观察到BHK（v-src）细胞提取物中的酪氨酸磷酸化活性高约五倍，这是由于src而非其他细胞酪氨酸激酶活性所致，因为与抗src血清预孵育会将活性降低至对照细胞水平。当表达v-src时，环磷酸腺苷依赖性蛋白激酶活性也发生了改变。从细胞的去污剂可溶部分解析出两种环磷酸腺苷依赖性同工酶，结果显示I型活性在BHK（v-src）细胞中选择性降低了约五倍。
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