与EcoRI核酸内切酶形成晶体复合物的扭结DNA。
Kinked DNA in crystalline complex with EcoRI endonuclease.
作者信息
Frederick C A, Grable J, Melia M, Samudzi C, Jen-Jacobson L, Wang B C, Greene P, Boyer H W, Rosenberg J M
出版信息
Nature. 1984;309(5966):327-31. doi: 10.1038/309327a0.
DOI:10.1038/309327a0
PMID:6328307
Abstract
The 3 A electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity. The DNA contains a torsional kink and other departures from the B conformation which unwind the DNA and thereby widen the major groove in the recognition site.
摘要
EcoRI核酸内切酶与寡核苷酸TCGCGAATTCGCG共结晶识别复合物的3A电子密度图显示，酶与DNA大沟之间紧密互补的界面是序列特异性的主要决定因素。该DNA含有一个扭转扭结以及其他与B构象的偏差，这些偏差使DNA解旋，从而加宽了识别位点的大沟。
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