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反向回旋酶——一种能将正向超螺旋引入DNA的拓扑异构酶。

Reverse gyrase--a topoisomerase which introduces positive superhelical turns into DNA.

作者信息

Kikuchi A, Asai K

出版信息

Nature. 1984;309(5970):677-81. doi: 10.1038/309677a0.

DOI:10.1038/309677a0
PMID:6328327
Abstract

An enzymatic activity which converts closed circular DNA into a positively supercoiled form is present in cellular extracts of Sulfolobus, an acidothermophilic archaebacterium. This novel enzyme is a type II DNA topoisomerase and is active only at temperatures greater than 55 degrees C, in the presence of ATP and Mg2+.

摘要

嗜酸嗜热古细菌硫磺叶菌的细胞提取物中存在一种能将闭环DNA转化为正超螺旋形式的酶活性。这种新型酶是一种II型DNA拓扑异构酶，仅在ATP和Mg2+存在的情况下，于温度高于55摄氏度时具有活性。

相似文献

1
Reverse gyrase--a topoisomerase which introduces positive superhelical turns into DNA.反向回旋酶——一种能将正向超螺旋引入DNA的拓扑异构酶。
Nature. 1984;309(5970):677-81. doi: 10.1038/309677a0.
2
Control of DNA topology during thermal stress in hyperthermophilic archaea: DNA topoisomerase levels, activities and induced thermotolerance during heat and cold shock in Sulfolobus.嗜热古菌热应激期间DNA拓扑结构的调控：嗜热栖热菌在热休克和冷休克期间的DNA拓扑异构酶水平、活性及诱导的耐热性
Mol Microbiol. 1999 Aug;33(4):766-77. doi: 10.1046/j.1365-2958.1999.01524.x.
3
Intrinsic DNA-dependent ATPase activity of reverse gyrase.反向回旋酶的内在依赖DNA的ATP酶活性。
J Biol Chem. 1987 Aug 5;262(22):10419-21.
4
ATP-dependent DNA topoisomerase from the archaebacterium Sulfolobus acidocaldarius. Relaxation of supercoiled DNA at high temperature.来自嗜热嗜酸硫化叶菌的ATP依赖性DNA拓扑异构酶。高温下超螺旋DNA的松弛。
J Mol Biol. 1984 Nov 5;179(3):559-63. doi: 10.1016/0022-2836(84)90080-9.
5
Analysis of DNA cleavage by reverse gyrase from Sulfolobus shibatae B12.来自嗜热栖热菌B12的反向回旋酶对DNA切割的分析。
Eur J Biochem. 1999 Feb;260(1):103-11. doi: 10.1046/j.1432-1327.1999.00128.x.
6
DNA topoisomerase III from extremely thermophilic archaebacteria. ATP-independent type I topoisomerase from Desulfurococcus amylolyticus drives extensive unwinding of closed circular DNA at high temperature.来自极端嗜热古细菌的DNA拓扑异构酶III。来自解淀粉脱硫球菌的不依赖ATP的I型拓扑异构酶在高温下驱动闭环DNA的大量解旋。
J Biol Chem. 1991 Jul 5;266(19):12321-8.
7
Positive supercoiling catalysed in vitro by ATP-dependent topoisomerase from Desulfurococcus amylolyticus.嗜淀粉脱硫球菌中依赖ATP的拓扑异构酶在体外催化的正超螺旋。
Eur J Biochem. 1988 Apr 15;173(2):395-9. doi: 10.1111/j.1432-1033.1988.tb14012.x.
8
DNA gyrase can supercoil DNA circles as small as 174 base pairs.DNA 回旋酶能够使小至174个碱基对的DNA环形成超螺旋结构。
EMBO J. 1989 Jun;8(6):1861-6. doi: 10.1002/j.1460-2075.1989.tb03582.x.
9
Studies on DNA polymerases and topoisomerases in archaebacteria.古细菌中DNA聚合酶和拓扑异构酶的研究。
Can J Microbiol. 1989 Jan;35(1):228-33. doi: 10.1139/m89-035.
10
A reverse gyrase with an unusual structure. A type I DNA topoisomerase from the hyperthermophile Methanopyrus kandleri is a two-subunit protein.一种具有独特结构的反向旋转酶。来自嗜热古菌坎氏甲烷嗜热菌的I型DNA拓扑异构酶是一种双亚基蛋白。
J Biol Chem. 1994 Apr 15;269(15):11081-9.

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