Kataoka Y, Gutman Y, Guidotti A, Panula P, Wroblewski J, Cosenza-Murphy D, Wu J Y, Costa E
Proc Natl Acad Sci U S A. 1984 May;81(10):3218-22. doi: 10.1073/pnas.81.10.3218.
Histochemical and biochemical studies demonstrate that gamma-aminobutyric acid (GABA), glutamic acid decarboxylase (EC 4.1.1.15), and GABA aminotransferase (EC 2.6.1.19) are present in bovine adrenal chromaffin cells. Moreover, [3H]GABA can be taken up and stored by primary cultures of adrenal chromaffin cells. Nicotinic receptor stimulation or KCl depolarization releases the [3H]GABA taken up by these cell cultures. GABA and benzodiazepine recognition sites located in chromaffin cells interact with each other with modalities similar to those described for GABA and benzodiazepine recognition sites located in synaptic membranes prepared from brain tissue. Bicuculline facilitates the release of catecholamine from chromaffin cells induced by nicotinic receptor stimulation but it fails to influence the release of catecholamine evoked by K+ depolarization. Since the GABA-benzodiazepine receptor system appears to modulate nicotinic receptor function, it is suggested that GABA transmission might participate in modulating responsiveness of chromaffin cells to incoming cholinergic stimuli.
组织化学和生物化学研究表明，γ-氨基丁酸（GABA）、谷氨酸脱羧酶（EC 4.1.1.15）和GABA转氨酶（EC 2.6.1.19）存在于牛肾上腺嗜铬细胞中。此外，[3H]GABA可被肾上腺嗜铬细胞原代培养物摄取并储存。烟碱样受体刺激或氯化钾去极化可释放这些细胞培养物摄取的[3H]GABA。嗜铬细胞中的GABA和苯二氮䓬识别位点相互作用，其方式类似于脑组织制备的突触膜中GABA和苯二氮䓬识别位点的相互作用方式。荷包牡丹碱促进烟碱样受体刺激诱导的嗜铬细胞释放儿茶酚胺，但不影响钾离子去极化诱发的儿茶酚胺释放。由于GABA-苯二氮䓬受体系统似乎调节烟碱样受体功能，则表明GABA传递可能参与调节嗜铬细胞对传入胆碱能刺激的反应性。
