不同胶原酶对从大鼠肝脏分离活肝细胞的影响。
Effect of different collagenases on the isolation of viable hepatocytes from rat liver.
作者信息
Queral A E, DeAngelo A B, Garrett C T
出版信息
Anal Biochem. 1984 Apr;138(1):235-7. doi: 10.1016/0003-2697(84)90794-2.
PMID:6329035
Abstract
The isolation of viable hepatocytes from rat liver was found to depend on the source of collagenase (EC 3.4.24.3) more than any other single factor examined. Collagenase purified from Achromobacter iophagus/Bacillus polymyxa (collagenase/dispase) gave reproducibly high viability without the use of complex perfusion protocols.
摘要
从大鼠肝脏中分离出有活力的肝细胞，发现其对胶原酶（EC 3.4.24.3）的来源依赖程度高于所检测的任何其他单一因素。从食油无色杆菌/多粘芽孢杆菌中纯化得到的胶原酶（胶原酶/分散酶），无需使用复杂的灌注方案就能持续产生高活力。
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