Rosa R, Calvin M C, Préhu M O, Lévi-Strauss M, Rosa J
Biochem Biophys Res Commun. 1984 May 16;120(3):715-20. doi: 10.1016/s0006-291x(84)80165-5.
Purified phosphoglyceromutase was hybridized in vitro with pure biphosphoglyceromutase . The hybrid showed an electrophoretic mobility identical to that of the intermediate band of red cell phosphoglyceromutase activity in the hemolysate patterns. Electrophoretic tests showed that the partially purified hybrid displayed both bisphosphoglyceromutase and phosphoglyceromutase activities and that the sample contained also a small portion of non-hybrid bisphosphoglyceromutase . By constrast to a non-hybrid mixture of the two purified mutases the hybrid exhibited heat instability of bisphosphoglyceromutase activity and neutralization of phosphoglyceromutase activity by anti- bisphosphoglyceromutase antibody.
将纯化的磷酸甘油变位酶与纯的二磷酸甘油变位酶进行体外杂交。杂交体显示出与溶血产物模式中红细胞磷酸甘油变位酶活性中间带相同的电泳迁移率。电泳试验表明，部分纯化的杂交体同时具有二磷酸甘油变位酶和磷酸甘油变位酶活性，并且样品中还含有一小部分非杂交的二磷酸甘油变位酶。与两种纯化变位酶的非杂交混合物相比，杂交体表现出二磷酸甘油变位酶活性的热不稳定性以及抗二磷酸甘油变位酶抗体对磷酸甘油变位酶活性的中和作用。
