来自大鼠胸腺的有丝分裂活跃淋巴细胞亚群中的1,25-二羟基维生素D3受体

1,25-Dihydroxyvitamin D3 receptors in a subset of mitotically active lymphocytes from rat thymus.

作者信息

Provvedini D M, Deftos L J, Manolagas S C

出版信息

Biochem Biophys Res Commun. 1984 May 31;121(1):277-83. doi: 10.1016/0006-291x(84)90719-8.

Abstract

Lymphocytes from the rat thymus gland were separated on Percoll gradient in two subsets. The first subset was enriched in large mitotically active cells and the second in small mitotically inert cells. The former cell preparation possessed a 3.3 S macromolecule with high affinity for 1,25-Dihydroxyvitamin D3 (Kd = 3.3 X 10(-10)M) which bound to DNA cellulose and was eluted from this resin with 0.26 M KCl. In contrast, the small-cell-enriched subset of thymic cells was negative for 1,25-Dihydroxyvitamin D3 specific binding. These findings support evidence from studies in human lymphocytes that there exists an association between mitotic activity and 1,25-Dihydroxyvitamin D3 receptor expression in this class of leukocytes.

摘要

大鼠胸腺淋巴细胞在Percoll梯度上被分离为两个亚群。第一个亚群富含具有高有丝分裂活性的大细胞，第二个亚群富含无有丝分裂活性的小细胞。前一种细胞制剂含有一种对1,25-二羟基维生素D3具有高亲和力的3.3 S大分子（解离常数Kd = 3.3×10⁻¹⁰M），它与DNA纤维素结合，并在0.26 M KCl中从该树脂上洗脱下来。相反，富含小细胞的胸腺细胞亚群对1,25-二羟基维生素D3特异性结合呈阴性。这些发现支持了对人类淋巴细胞研究的证据，即这类白细胞的有丝分裂活性与1,25-二羟基维生素D3受体表达之间存在关联。