Tei I, Makino Y, Kadofuku T, Kanamaru I, Konno K
Biochem Biophys Res Commun. 1984 Jun 15;121(2):717-21. doi: 10.1016/0006-291x(84)90240-7.
The change of transferrin receptors in regenerating rat liver cells after partial hepatectomy was demonstrated. The binding of 125I-labeled transferrin to the cells began to increase after 24 h of partial hepatectomy and reached about double that of the non-operated normal rat liver cells. A Scatchard analysis of binding parameters showed 1.62 X 10(5) (Ka: 1.04 X 10(7) M-1) in normal cells and 3.36 X 10(5) (Ka: 1.23 X 10(7) M-1) in regenerating cells. This reflected on increase of transferrin receptor number and little change occurred in the binding affinity between transferrin and its surface receptor.
证明了部分肝切除术后再生大鼠肝细胞中转铁蛋白受体的变化。部分肝切除术后24小时，125I标记的转铁蛋白与细胞的结合开始增加，达到未手术正常大鼠肝细胞的约两倍。结合参数的Scatchard分析显示，正常细胞中为1.62×10(5)(Ka: 1.04×10(7) M-1)，再生细胞中为3.36×10(5)(Ka: 1.23×10(7) M-1)。这反映了转铁蛋白受体数量的增加，并且转铁蛋白与其表面受体之间的结合亲和力几乎没有变化。
