南印度女性羊水的抗菌活性

Antimicrobial activity of amniotic fluid in South Indian women.

作者信息

Mathai M, Jairaj P, Thangavelu C P, Mathai E, Balasubramaniam N

出版信息

Br J Obstet Gynaecol. 1984 Jun;91(6):560-4. doi: 10.1111/j.1471-0528.1984.tb04803.x.

DOI:10.1111/j.1471-0528.1984.tb04803.x

Abstract

Samples of amniotic fluid obtained from 48 South Indian women in the third trimester of pregnancy were studied for antimicrobial activity. The growth of Staphylococcus albus, Candida albicans and Clostridium perfringens was inhibited by nearly all samples studied while the growth of Staphylococcus aureus, Escherichia coli and Bacteroides fragilis was inhibited by 50%, 42% and 18% of samples respectively. The growth of Streptococcus faecalis was not inhibited. Using radial immunodiffusion, IgG was measurable in all 10 samples studied (mean 23 mg/dl), whereas IgA was measurable in only three of these samples (mean 1.32 mg/dl). However, while specific IgA against C. albicans was detected by indirect immunofluorescence in 93% of samples, specific IgG against C. albicans was detected in only 26% of samples (P less than 0.001). Amniotic fluid obtained from parous women had greater inhibitory activity against E. coli (P less than 0.05) than did the amniotic fluid obtained from nulliparae.

摘要

对48名处于妊娠晚期的南印度妇女获取的羊水样本进行了抗菌活性研究。几乎所有研究样本均抑制了白色葡萄球菌、白色念珠菌和产气荚膜梭菌的生长，而金黄色葡萄球菌、大肠杆菌和脆弱拟杆菌的生长分别被50%、42%和18%的样本所抑制。粪肠球菌的生长未受到抑制。采用放射免疫扩散法，在所研究的全部10个样本中均检测到了IgG（平均23mg/dl），而其中只有3个样本检测到了IgA（平均1.32mg/dl）。然而，通过间接免疫荧光法在93%的样本中检测到了针对白色念珠菌的特异性IgA，而仅在26%的样本中检测到了针对白色念珠菌的特异性IgG（P小于0.001）。经产妇的羊水对大肠杆菌的抑制活性（P小于0.05）高于初产妇的羊水。