Meyer J L, Fleisch H
Biochim Biophys Acta. 1984 Jun 15;799(2):115-21. doi: 10.1016/0304-4165(84)90284-8.
Rat and human serum and plasma were shown to contain considerable amounts of calcium phosphate precipitation inhibitors. Two general classes of inhibiting molecules were observed for both species: high molecular weight (approx. 30 000-200 000) and low molecular weight (less than 1000). The high molecular weight components eluted from a Bio-Gel P-200 column in two peaks, one at approx. 158 000 and a broader peak at approx. 43 000. The identity of these inhibitors is unknown at present. Low molecular weight inhibitors include magnesium, pyrophosphate, and citrate ions and at least one unidentified component that coelutes with pyrophosphate and citrate on a Bio-Gel P-4 column. Quantitatively, most of the inhibitor activity resides in the high molecular weight components and it is possible that it is this activity which is responsible for maintaining the metastability of the circulating fluids. The role of the low molecular weight components may be to regulate calcification at sites inaccessible to high molecular weight molecules.
已证明大鼠和人类的血清及血浆中含有大量磷酸钙沉淀抑制剂。在这两个物种中均观察到两类抑制分子：高分子量（约30000 - 200000）和低分子量（小于1000）。从Bio - Gel P - 200柱上洗脱的高分子量组分出现两个峰，一个在约158000处，另一个较宽的峰在约43000处。目前这些抑制剂的具体成分尚不清楚。低分子量抑制剂包括镁离子、焦磷酸根离子和柠檬酸根离子，以及至少一种在Bio - Gel P - 4柱上与焦磷酸根和柠檬酸根共洗脱的未鉴定成分。从数量上看，大部分抑制活性存在于高分子量组分中，可能正是这种活性维持了循环液的亚稳定性。低分子量组分的作用可能是在高分子量分子无法到达的部位调节钙化。
