Garris D R, Ingenito A J, McConnaughey M M, Dar M S
Biol Reprod. 1984 May;30(4):863-8. doi: 10.1095/biolreprod30.4.863.
The effects of estradiol (1 microgram: E-1) treatment on uterine hyperemia and uterine sensitivity to various biogenic compounds were evaluated in ovariectomized (OVX) animals treated with either sesame oil or E-1 for 3 days. The E-1 treatments induced significant elevations in uterine weight, blood flow, and alpha- and beta-receptor numbers as compared with oil-treated controls. In contrast, uterine norepinephrine (NE) levels were reduced in E-1-treated, OVX guinea pigs as compared with oil-treated controls. Uterine sensitivity and responsivity to NE (10(-6) M) and acetylcholine (ACH: 10(-8) M) were either comparable to, or enhanced, in E-1-treated animals as compared with controls. In particular, combined ACH-NE treatment induced a dramatic increase in contraction force in E-1-treated uteri as compared with uteri from oil-treated animals. The use of specific adrenergic alpha- (phentolamine: 10(-6) M) or beta- (propranolol: 10(-6) M) receptor blocking agents indicated that the estrogenic response was mediated via the alpha-adrenergic receptor complex. Since atropine (10(-8) M) effectively blocked the cholinergic accentuation of this uterine response, it is suggested that a cholinergic priming, or beta-receptor block, is necessary for the full expression of the alpha-adrenergic-mediated, estrogenic response in the guinea pig. The estrogen-associated increase in available alpha- and beta-receptors and depressed tissue NE levels probably account for both the hyperemic response and enhanced tissue sensitivity to biogenic compounds in the guinea pig.
在用芝麻油或雌二醇（E-1）处理3天的去卵巢（OVX）动物中，评估了雌二醇（1微克：E-1）处理对子宫充血以及子宫对各种生物活性化合物敏感性的影响。与用油处理的对照组相比，E-1处理导致子宫重量、血流量以及α和β受体数量显著增加。相反，与用油处理的对照组相比，用E-1处理的OVX豚鼠子宫去甲肾上腺素（NE）水平降低。与对照组相比，E-1处理的动物子宫对NE（10⁻⁶ M）和乙酰胆碱（ACH：10⁻⁸ M）的敏感性和反应性相当或增强。特别是，与用油处理的动物的子宫相比，联合使用ACH-NE处理可使E-1处理的子宫收缩力显著增加。使用特异性肾上腺素能α（酚妥拉明：10⁻⁶ M）或β（普萘洛尔：10⁻⁶ M）受体阻断剂表明，雌激素反应是通过α-肾上腺素能受体复合物介导的。由于阿托品（10⁻⁸ M）有效地阻断了这种子宫反应的胆碱能增强作用，因此提示胆碱能启动或β受体阻断对于豚鼠中α-肾上腺素能介导的雌激素反应的充分表达是必要的。雌激素相关的可用α和β受体增加以及组织NE水平降低可能解释了豚鼠的充血反应和组织对生物活性化合物敏感性增强。
