从胎鼠棕色脂肪组织制备激素敏感性脂肪细胞。
Preparation of hormone-sensitive adipocytes from foetal rat brown adipose tissue.
作者信息
Elliott K R, Roncero C, Fernandez M, Benito M
出版信息
Biosci Rep. 1984 May;4(5):397-401. doi: 10.1007/BF01122504.
PMID:6329353
Abstract
A collagenase-dispersion method involving Percoll gradient separation is described for the production of adipocytes from foetal rat brown adipose tissue. The cells produced are hormone-sensitive as judged by cAMP accumulation and lipolysis.
摘要
描述了一种涉及Percoll梯度分离的胶原酶分散方法，用于从胎鼠棕色脂肪组织中产生脂肪细胞。通过环磷酸腺苷（cAMP）积累和脂解作用判断，所产生的细胞对激素敏感。
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