Miyazaki K, Yashiro T, Suzuki T
Endocrinol Jpn. 1984 Feb;31(1):23-32. doi: 10.1507/endocrj1954.31.23.
An electron microscopic study was performed to clarify the effects of tunicamycin, a glycosylation inhibitor, on rat anterior pituitary cells. Tunicamycin (10, 50, and 100 micrograms/250 g B.W.) was intraperitoneally injected into rats, which were sacrificed 24 hrs later. Protein hormone producing GH and prolactin cells, and ACTH cells which are known to have a glycosylated precursor, showed no recognizable ultrastructural changes. TSH cells and gonadotrophs, both of which secrete glycoprotein hormones consisting of alpha and beta subunits, showed remarkable dilatation of the rough endoplasmic reticulum, and decreased numbers of secretory granules. These results suggest that the role of glycosylation in TSH cells and gonadotrophs may have a different biological significance from that in ACTH cells.
进行了一项电子显微镜研究，以阐明糖基化抑制剂衣霉素对大鼠垂体前叶细胞的影响。将衣霉素（10、50和100微克/250克体重）腹腔注射到大鼠体内，24小时后处死大鼠。产生蛋白质激素的生长激素和催乳素细胞，以及已知具有糖基化前体的促肾上腺皮质激素细胞，未显示出可识别的超微结构变化。促甲状腺激素细胞和促性腺激素细胞，两者均分泌由α和β亚基组成的糖蛋白激素，显示粗面内质网明显扩张，分泌颗粒数量减少。这些结果表明，糖基化在促甲状腺激素细胞和促性腺激素细胞中的作用可能与在促肾上腺皮质激素细胞中的作用具有不同的生物学意义。
