Zak R A, Frenkiel P G, Marks J W, Bonorris G G, Allen A, Schoenfield L J
Gastroenterology. 1984 Aug;87(2):263-9.
Male prairie dogs received in standard diets either 0.08% cholesterol (control, n = 30) or 1.2% cholesterol (lithogenic, n = 31). Animals were killed at days 2-4, 7, 10, 21, and 39 to determine the temporal sequence of changes in mucosal cyclic adenosine 3':5'-monophosphate in the gallbladder and of cholesterol saturation, glycoproteins, cholesterol crystals, and gallstones in bile of prairie dogs fed a cholesterol-rich lithogenic diet. Glycoprotein concentration in bile in the lithogenic group was significantly elevated compared to controls on all days of death. Saturation of bile and formation of cholesterol crystals occurred only in the lithogenic group, detected first after 7 days of feeding. Gallstones were found in the lithogenic group only. Elevation of cyclic adenosine 3':5'-monophosphate in the mucosa of gallbladders was found in the lithogenic group only, beginning at day 10. In summary, increased glycoproteins in bile preceded cholesterol saturation and crystallization which, in turn, preceded increased mucosal cyclic adenosine 3':5'-monophosphate.
雄性草原犬鼠被给予标准饮食，其中一组含0.08%胆固醇（对照组，n = 30），另一组含1.2%胆固醇（致石性饮食组，n = 31）。在第2 - 4天、第7天、第10天、第21天和第39天处死动物，以确定喂食富含胆固醇的致石性饮食的草原犬鼠胆囊黏膜中环磷酸腺苷（cAMP）的变化时间顺序，以及胆汁中胆固醇饱和度、糖蛋白、胆固醇晶体和胆结石的变化时间顺序。在所有处死日，致石性饮食组胆汁中的糖蛋白浓度均显著高于对照组。胆汁饱和及胆固醇晶体形成仅发生在致石性饮食组，在喂食7天后首次检测到。胆结石仅在致石性饮食组中发现。胆囊黏膜中的环磷酸腺苷升高仅见于致石性饮食组，始于第10天。总之，胆汁中糖蛋白增加先于胆固醇饱和及结晶，而胆固醇饱和及结晶又先于黏膜中环磷酸腺苷增加。
