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新型克隆载体的构建与特性分析。VII. 质粒pBR327par的构建，pBR327的一个完全测序的稳定衍生物，含有pSC101的par位点。

Construction and characterization of new cloning vehicles. VII. Construction of plasmid pBR327par, a completely sequenced, stable derivative of pBR327 containing the par locus of pSC101.

作者信息

Zurita M, Bolivar F, Soberón X

出版信息

Gene. 1984 Apr;28(1):119-22. doi: 10.1016/0378-1119(84)90094-5.

DOI:10.1016/0378-1119(84)90094-5
PMID:6329912
Abstract

In vitro recombinant DNA experiments, using plasmid pBR327 and a DNA fragment derived from plasmid pSC101 containing the par region, resulted in the construction of plasmid pBR327par. This new cloning vehicle has all the cloning properties of the parental plasmid, and is more stable than pBR327. Since the nucleotide sequence of the par region has been determined, this new vector is completely characterized. Some features of the sequence with possible functional significance are discussed.

摘要

利用质粒pBR327和来自含有par区域的质粒pSC101的DNA片段进行的体外重组DNA实验，构建出了质粒pBR327par。这种新的克隆载体具有亲本质粒的所有克隆特性，并且比pBR327更稳定。由于par区域的核苷酸序列已经确定，这种新载体已得到全面表征。文中讨论了该序列一些可能具有功能意义的特征。

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Gene. 1984 Apr;28(1):119-22. doi: 10.1016/0378-1119(84)90094-5.
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