大肠杆菌K-12色氨酸特异性通透酶结构基因mtr的克隆、核苷酸序列及特性分析
Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12.
作者信息
Heatwole V M, Somerville R L
机构信息
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
出版信息
J Bacteriol. 1991 Jan;173(1):108-15. doi: 10.1128/jb.173.1.108-115.1991.
Abstract
The mtr gene of Escherichia coli K-12 encodes an L-tryptophan-specific permease. This gene was originally identified through the isolation of mutations in the 69-min region of the chromosome, closely linked to argG. Cells with lesions in mtr display a phenotype of 5-methyltryptophan resistance. The mtr gene was cloned by using the mini-Mu system. The amino acid sequence of Mtr (414 codons), deduced by DNA sequence analysis, was found to be 33% identical to that of another single-component transport protein, the tyrosine-specific permease, TyrP. The hydropathy plots of the two permeases were similar. Possible operator sites for the tyrosine and tryptophan repressors are situated within the region of DNA that is likely to be the mtr promoter.
摘要
大肠杆菌K-12的mtr基因编码一种L-色氨酸特异性通透酶。该基因最初是通过分离染色体69分钟区域内与argG紧密连锁的突变而鉴定出来的。mtr基因发生损伤的细胞表现出对5-甲基色氨酸的抗性表型。利用mini-Mu系统克隆了mtr基因。通过DNA序列分析推导得出的Mtr氨基酸序列（414个密码子）与另一种单组分转运蛋白——酪氨酸特异性通透酶TyrP的氨基酸序列有33%的同一性。这两种通透酶的亲水性图谱相似。酪氨酸和色氨酸阻遏物的可能操纵位点位于可能是mtr启动子的DNA区域内。
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