大肠杆菌中一种大环内酯抗性-β-半乳糖苷酶（ermC-lacZ）基因融合体的调控
Regulation of a macrolide resistance-beta-galactosidase (ermC-lacZ) gene fusion in Escherichia coli.
作者信息
Kirsch D R, Lai M H
出版信息
J Bacteriol. 1984 Jul;159(1):381-4. doi: 10.1128/jb.159.1.381-384.1984.
Abstract
A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ermC gene in addition to the fusion produced lower levels of beta-galactosidase, suggesting that the ermC gene product exerts negative feedback control on expression.
摘要
将假定的衰减子加上ermC（红霉素抗性）结构基因的前219个核苷酸与5'末端缺失的lacZ基因构建的融合体产生了中等水平的基础β-半乳糖苷酶，添加红霉素后该酶水平升高。另一个构建体除了融合体之外还包含完整的ermC基因，其产生的β-半乳糖苷酶水平较低，这表明ermC基因产物对表达施加负反馈控制。
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