Pegg-Feige K, Doane F W
J Virol Methods. 1983 Dec;7(5-6):315-9. doi: 10.1016/0166-0934(83)90084-8.
The type of grid support film used in solid phase immunoelectron microscopy (SPIEM) for poliovirus affected the efficiency of virus trapping. Parlodion/carbon films coated with homologous antiserum were found to be 9 times more efficient in trapping virus than similar grids prepared with Formvar/carbon, and 20 times better than SPIEM grids prepared with pure carbon films. Trapping efficiency could be further improved if support films were pretreated prior to exposure to viral antiserum. Maximum efficiency was obtained with Parlodion/carbon films pretreated with UV light. Almost as efficient were Parlodion/carbon and Formvar/carbon films pretreated with glow discharge ionization.
用于脊髓灰质炎病毒的固相免疫电子显微镜（SPIEM）中所使用的网格支撑膜类型影响病毒捕获效率。发现涂有同源抗血清的火棉胶/碳膜捕获病毒的效率比用福尔马林/碳制备的类似网格高9倍，比用纯碳膜制备的SPIEM网格高20倍。如果在暴露于病毒抗血清之前对支撑膜进行预处理，捕获效率可以进一步提高。用紫外线预处理的火棉胶/碳膜可获得最大效率。用辉光放电电离预处理的火棉胶/碳膜和福尔马林/碳膜几乎同样有效。
