Snowden E U, Khan-Dawood F S, Dawood M Y
J Clin Endocrinol Metab. 1984 Aug;59(2):298-302. doi: 10.1210/jcem-59-2-298.
To determine the influence of ovarian sex steroid hormones on endogenous opioid regulation of pituitary FSH, LH, and PRL secretion, six women were studied during the follicular phase (days 8-9) and luteal phase (days 21-23) of their menstrual cycles. An iv bolus dose of 10 mg of the opiate antagonist naloxone was given, and plasma FSH, LH, and PRL were measured at -30, -15, 0, 15, 30, 45, 60, 90, 120, and 180 min. During the follicular phase, baseline plasma FSH and LH levels were 10.7 +/- 0.9 and 16.7 n+/- 2.0 mIU/ml (mean +/- SEM), respectively; the plasma PRL level was 11.7 +/- 1.2 ng/ml. Naloxone did not significantly alter plasma FSH, LH, or PRL during the follicular phase. Basal levels of LH were significantly lower during the luteal phase than during the follicular phase (P less than 0.01). During the luteal phase, plasma LH increased significantly from a basal level of 10.0 +/- 1.0 to 20.8 +/- 3.0 mIU at 30 min (P less than 0.001) and remained significantly elevated at 90 min. Similarly, plasma PRL increased significantly from a basal level of 11.0 +/- 0.7 to 16.2 +/- 2.7 ng/ml at 30 min (P less than 0.025), but decreased by 90 min to 12.5 +/- 1.5 ng/ml. Plasma FSH did not change after naloxone treatment. Our results suggest that endogenous opiates have a prominent inhibitory effect on pituitary gonadotropin and PRL secretion only during the luteal phase of the menstrual cycle.
为了确定卵巢性甾体激素对垂体促卵泡激素（FSH）、促黄体生成素（LH）和催乳素（PRL）分泌的内源性阿片肽调节的影响，对6名女性在其月经周期的卵泡期（第8 - 9天）和黄体期（第21 - 23天）进行了研究。静脉注射10毫克阿片拮抗剂纳洛酮，在 - 30、 - 15、0、15、30、45、60、90、120和180分钟时测量血浆FSH、LH和PRL。在卵泡期，血浆FSH和LH的基线水平分别为10.7±0.9和16.7±2.0 mIU/ml（平均值±标准误）；血浆PRL水平为11.7±1.2 ng/ml。在卵泡期，纳洛酮未显著改变血浆FSH、LH或PRL。黄体期LH的基础水平显著低于卵泡期（P < 0.01）。在黄体期，血浆LH在30分钟时从基础水平10.0±1.0显著增加至20.8±3.0 mIU（P < 0.001），并在90分钟时仍显著升高。同样，血浆PRL在30分钟时从基础水平11.0±0.7显著增加至16.2±2.7 ng/ml（P < 0.025），但在90分钟时降至12.5±1.5 ng/ml。纳洛酮治疗后血浆FSH未改变。我们的结果表明，内源性阿片肽仅在月经周期的黄体期对垂体促性腺激素和PRL分泌有显著的抑制作用。
