Boehm T L, Drahovsky D
J Biochem Biophys Methods. 1984 May;9(2):153-61. doi: 10.1016/0165-022x(84)90006-x.
We have studied with a number of bacterial restriction enzymes the conditions for digestion of DNA in agarose and polyacrylamide gels. The restriction endonucleases HpaII, MspI, HaeIII, HindIII, TaqI, HhaI, AluI, BamHI, EcoRI and SalI are capable of digesting DNA in agarose gels of low electroendosmosis and low sulfate concentration. All enzymes, except BamHI, are also capable of digesting DNA in polyacrylamide gels. With this method, rapid two-dimensional restriction mapping of genomes with low and high sequence complexity is possible.
我们用多种细菌限制酶研究了在琼脂糖凝胶和聚丙烯酰胺凝胶中消化DNA的条件。限制性内切酶HpaII、MspI、HaeIII、HindIII、TaqI、HhaI、AluI、BamHI、EcoRI和SalI能够在低电渗和低硫酸盐浓度的琼脂糖凝胶中消化DNA。除BamHI外，所有酶也都能够在聚丙烯酰胺凝胶中消化DNA。用这种方法，对低序列复杂性和高序列复杂性的基因组进行快速二维限制酶切图谱分析是可行的。
