Hérissé J, Courtois G, Galibert F
Gene. 1978 Dec;4(4):279-94. doi: 10.1016/0378-1119(78)90046-x.
Hydrolysis of the EcoRI F fragment of the adenovirus 2 genome with HaeIII, HpaII and AluI restriction enzymes gives respectively 9, 11, and 11 fragments, the size of which ranges from 20 bp for the smallest HpaII k fragment to 585 for the largest AluI A fragment. The order of fragments was mainly deduced from partial hydrolysis analyses. The relative order of all restriction sites and the distance in nucleotides between them were obtained through secondary analyses of each restriction fragment by the two other enzymes. Position of the KpnI, HindIII, MboI and SmaI sites within the EcoRI F fragment were also reevaluated.
用HaeIII、HpaII和AluI限制性内切酶对腺病毒2基因组的EcoRI F片段进行水解，分别产生9、11和11个片段，其大小范围从最小的HpaII k片段的20碱基对到最大的AluI A片段的585碱基对。片段的顺序主要通过部分水解分析推导得出。通过用另外两种酶对每个限制性片段进行二次分析，获得了所有限制性位点的相对顺序及其之间的核苷酸距离。还重新评估了EcoRI F片段内KpnI、HindIII、MboI和SmaI位点的位置。
