Brain S, Camp R, Dowd P, Black A K, Greaves M
J Invest Dermatol. 1984 Jul;83(1):70-3. doi: 10.1111/1523-1747.ep12261712.
The 5,12-dihydroxy metabolite of arachidonic acid, leukotriene B4, is a highly potent neutrophil chemoattractant. In view of the characteristic epidermal neutrophil infiltrate in psoriasis, the presence of leukotriene B4 in samples from untreated lesional and uninvolved skin has been sought. Chambers were fixed to abraded skin and filled with phosphate-buffered saline (PBS). After 35 min, the fluid was removed, and acidic lipids were extracted and subjected to high-performance liquid chromatography (HPLC). Extracts were purified by both straight- and reversed-phase HPLC, and assay of evaporated fractions by an agarose microdroplet chemokinesis technique indicated the presence of leukotriene B4-like material. No significant leukotriene B4-like activity was found in samples from uninvolved skin. Subsequent experiments using a modification of the initial skin chamber method indicated that leukotriene B4 was being released from deeper layers of lesional skin and not only from superficial scale. Monohydroxy-eicosatetraenoic acid-like activity was also seen in lesional samples as determined by straight-phase HPLC and chemokinesis assay. These findings and the proinflammatory properties of these compounds in human skin suggest that they may play a role in the pathogenesis of the psoriatic neutrophil infiltrate.
花生四烯酸的5,12 - 二羟基代谢产物白三烯B4是一种高效的中性粒细胞趋化因子。鉴于银屑病特征性的表皮中性粒细胞浸润，研究人员探寻了未经治疗的皮损和非皮损皮肤样本中白三烯B4的存在情况。将小室固定于磨损的皮肤上，并注入磷酸盐缓冲盐水（PBS）。35分钟后，吸出液体，提取酸性脂质并进行高效液相色谱（HPLC）分析。提取物通过正相和反相HPLC进行纯化，采用琼脂糖微滴趋化技术对蒸发组分进行分析，结果表明存在白三烯B4样物质。在非皮损皮肤样本中未发现明显的白三烯B4样活性。随后采用对初始皮肤小室方法进行改良的实验表明，白三烯B4是从皮损皮肤的深层释放出来的，而不仅仅是从表层鳞屑中释放。通过正相HPLC和趋化分析测定，在皮损样本中还观察到单羟基 - 二十碳四烯酸样活性。这些发现以及这些化合物在人体皮肤中的促炎特性表明，它们可能在银屑病中性粒细胞浸润的发病机制中发挥作用。
