Camp R D, Mallet A I, Woollard P M, Brain S D, Black A K, Greaves M W
Prostaglandins. 1983 Sep;26(3):431-47. doi: 10.1016/0090-6980(83)90178-8.
Psoriasis is a common chronic inflammatory and proliferative skin disease characterised by epidermal neutrophil infiltration which may be induced by chemotactic substances in the involved epidermis. Superficial psoriatic scale was shown to contain biologically active amounts of leukotriene B4 and monohydroxy-eicosatetraenoic acid (HETE)-like material as determined by assay for chemokinetic activity in high performance liquid chromatography (HPLC) fractions of scale extracts. Extracts of scale and chamber fluid from abraded lesional and uninvolved psoriatic skin were purified by HPLC and appropriate fractions were analysed by gas chromatography - mass spectrometry (GC-MS). The following monohydroxy metabolites of arachidonic, linoleic and 11,14-eicosadienoic acids were identified: 15-HETE, 12-HETE, 11-HETE, 9-HETE, 8-HETE, 5-HETE, 13-hydroxy-octadecadienoic acid (13-HODD), 9-HODD and 15-hydroxy-eicosadienoic acid (15-HEDE). The results suggested that 12-HETE, 13-HODD and 9-HODD are the most abundant monohydroxy fatty acids in the psoriatic skin extracts described above. Assays of 13-HODD, 9-HODD and 15-HEDE for chemokinetic activity were negative with concentrations up to 10(-4)M. The biological significance of these three compounds in not known, but some of the hydroxylated metabolites of arachidonic acid may, by virtue of their chemotactic properties, be relevant to the pathogenesis of the psoriatic neutrophil infiltrate.
银屑病是一种常见的慢性炎症性增殖性皮肤病，其特征为表皮中性粒细胞浸润，这可能由受累表皮中的趋化物质诱导。通过对鳞屑提取物的高效液相色谱（HPLC）馏分中的化学动力学活性进行测定，发现浅表性银屑病鳞屑含有生物活性量的白三烯B4和单羟基 - 二十碳四烯酸（HETE）样物质。通过HPLC对来自擦伤的皮损和未受累的银屑病皮肤的鳞屑和腔室液提取物进行纯化，并通过气相色谱 - 质谱联用（GC-MS）分析适当的馏分。鉴定出了花生四烯酸、亚油酸和11,14 - 二十碳二烯酸的以下单羟基代谢产物：15 - HETE、12 - HETE、11 - HETE、9 - HETE、8 - HETE、5 - HETE、13 - 羟基 - 十八碳二烯酸（13 - HODD）、9 - HODD和15 - 羟基 - 二十碳二烯酸（15 - HEDE）。结果表明，12 - HETE、13 - HODD和9 - HODD是上述银屑病皮肤提取物中最丰富的单羟基脂肪酸。对浓度高达10^(-4)M的13 - HODD、9 - HODD和15 - HEDE进行化学动力学活性测定呈阴性。这三种化合物的生物学意义尚不清楚，但花生四烯酸的一些羟基化代谢产物可能因其趋化特性而与银屑病中性粒细胞浸润的发病机制有关。
