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从金黄色葡萄球菌中提取质粒DNA的快速微量方法。

Rapid microprocedure for extraction of plasmid DNA from Staphylococcus aureus.

作者信息

Dunkle L M, Sippel C J

出版信息

J Infect Dis. 1984 Jun;149(6):921-3. doi: 10.1093/infdis/149.6.921.

DOI:10.1093/infdis/149.6.921
PMID:6330238
Abstract

A rapid micromethod for extraction of plasmid DNA from Staphylococcus aureus in quantities sufficient for restriction endonuclease analysis was developed. The method of "fingerprinting" is useful for identification of epidemic strains in the clinical setting and is more reliable than frequently used determinations of plasmid molecular weight, particularly when small numbers of plasmids are involved.

摘要

已开发出一种快速微量方法,可从金黄色葡萄球菌中提取足够量的质粒DNA用于限制性内切酶分析。“指纹图谱”方法有助于在临床环境中鉴定流行菌株,并且比常用的质粒分子量测定方法更可靠,尤其是在涉及少量质粒时。

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Rapid microprocedure for extraction of plasmid DNA from Staphylococcus aureus.从金黄色葡萄球菌中提取质粒DNA的快速微量方法。
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引用本文的文献

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J Clin Microbiol. 1994 Nov;32(11):2635-40. doi: 10.1128/jcm.32.11.2635-2640.1994.
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Restriction enzyme analysis of plasmid DNA and bacteriophage typing of paired Staphylococcus aureus blood culture isolates.对质粒DNA进行限制性内切酶分析,并对配对的金黄色葡萄球菌血培养分离株进行噬菌体分型。
J Clin Microbiol. 1989 Aug;27(8):1874-9. doi: 10.1128/jcm.27.8.1874-1879.1989.
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Outbreaks of infection with methicillin-resistant Staphylococcus aureus on neonatal and burns units of a new hospital.
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Epidemiol Infect. 1990 Oct;105(2):215-28. doi: 10.1017/s0950268800047828.