Doebbeling B N, Pfaller M A, Hollis R J, Boyken L D, Pignatari A C, Herwaldt L A, Wenzel R P
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.
Eur J Clin Microbiol Infect Dis. 1992 Jan;11(1):4-8. doi: 10.1007/BF01971263.
Staphylococcus aureus isolates (n = 1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13% of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of greater than 10% on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.
对来自欧洲、美国和巴西20个中心的1201株金黄色葡萄球菌分离株进行了流行病学标志物检测。质粒分型和质粒DNA的限制性内切酶分析证实，13%的临床分离株中存在一种明显相同的质粒。在所有研究的20家医院中均发现了该质粒，在三大洲的每家医院中其总体出现频率均超过10%。由于流行病学上不相关的菌株可能共享的葡萄球菌质粒相对较少，因此这项原本有用的技术存在固有局限性。此外，这些数据表明，在进行分子分型方法时，纳入来自当地的不相关金黄色葡萄球菌菌株作为对照非常重要。
