人肾小球内皮细胞的分离、鉴定及体外培养
Isolation, characterization, and propagation in vitro of human glomerular endothelial cells.
作者信息
Striker G E, Soderland C, Bowen-Pope D F, Gown A M, Schmer G, Johnson A, Luchtel D, Ross R, Striker L J
出版信息
J Exp Med. 1984 Jul 1;160(1):323-8. doi: 10.1084/jem.160.1.323.
Abstract
Human glomerular endothelial cells have been isolated, cloned, and characterized. They appeared as the first outgrowth from human glomeruli in the presence of platelet-derived growth factor, which was also a requirement for continuous growth. By phase microscopy they appeared as monolayers of polygonal cells. Von Willebrand's factor (VWF) was detected in the cytoplasm of all clones. Their intermediate filaments differed antigenically from that present in human umbilical vein endothelial cells. Like other endothelial cells, they demonstrated high levels of membrane-associated angiotensin-converting enzyme (ACE).
摘要
人肾小球内皮细胞已被分离、克隆和鉴定。在血小板衍生生长因子存在的情况下，它们是最早从人肾小球长出的细胞，血小板衍生生长因子也是细胞持续生长所必需的。通过相差显微镜观察，它们呈现为多边形细胞单层。在所有克隆细胞的细胞质中都检测到了血管性血友病因子（VWF）。它们的中间丝在抗原性上与人脐静脉内皮细胞中的不同。与其他内皮细胞一样，它们表现出高水平的膜相关血管紧张素转换酶（ACE）。
相似文献
1
Isolation, characterization, and propagation in vitro of human glomerular endothelial cells.人肾小球内皮细胞的分离、鉴定及体外培养
J Exp Med. 1984 Jul 1;160(1):323-8. doi: 10.1084/jem.160.1.323.
2
Regulation of bovine glomerular endothelial cell growth in vitro.
Am J Physiol. 1989 Jan;256(1 Pt 1):C182-9. doi: 10.1152/ajpcell.1989.256.1.C182.
3
Separation of luminal and abluminal membrane enriched domains from cultured bovine aortic endothelial cells: monoclonal antibodies specific for endothelial cell plasma membranes.
Biochim Biophys Acta. 1987 Mar 26;898(1):37-52. doi: 10.1016/0005-2736(87)90107-6.
4
Ontogenesis of angiotensin-I converting enzyme in human kidney.人肾脏中血管紧张素-I转换酶的个体发生
Kidney Int. 1987 Nov;32(5):684-90. doi: 10.1038/ki.1987.261.
5
Isolation and culture of endothelial cells from the lungs of small animals.
Am Rev Respir Dis. 1979 Jun;119(6):853-68. doi: 10.1164/arrd.1979.119.6.853.
6
Inbred guinea pig aortic endothelial cell clones. Model for studying the vascular endothelium under totally isologous conditions.
Lab Invest. 1986 Dec;55(6):703-16.
7
Establishment and characterization of an immortalized bovine glomerular endothelial cell line.永生化牛肾小球内皮细胞系的建立与鉴定
Nihon Jinzo Gakkai Shi. 1994 Aug;36(8):883-9.
8
Angiotensin-converting enzyme in non-neoplastic kidney diseases.非肿瘤性肾脏疾病中的血管紧张素转换酶
Kidney Int. 1999 Oct;56(4):1442-54. doi: 10.1046/j.1523-1755.1999.00660.x.
9
Varicose veins as a source of adult human endothelial cells.静脉曲张作为成人内皮细胞的来源。
Tissue Cell. 1985;17(2):171-6. doi: 10.1016/0040-8166(85)90086-2.
10
Human endothelial cells in vitro.体外培养的人内皮细胞。
Methods Cell Biol. 1980;21A:135-51. doi: 10.1016/s0091-679x(08)60763-3.
引用本文的文献
1
Enzymatic processing of angiotensin peptides by human glomerular endothelial cells.人肾小球内皮细胞对血管紧张肽的酶促加工。
Am J Physiol Renal Physiol. 2012 Jun 15;302(12):F1583-94. doi: 10.1152/ajprenal.00087.2012. Epub 2012 Mar 28.
2
Cell culture from rat renal glomeruli.
Virchows Arch. 1994;425(4):391-8. doi: 10.1007/BF00189577.
3
Production of platelet-derived growth factorlike protein by rat mesangial cells in culture.培养的大鼠系膜细胞产生血小板衍生生长因子样蛋白。
J Clin Invest. 1987 Sep;80(3):675-83. doi: 10.1172/JCI113121.
4
Mesangial cell hillocks. Nodular foci of exaggerated growth of cells and matrix in prolonged culture.系膜细胞小丘。长期培养中细胞和基质过度生长的结节状病灶。
Am J Pathol. 1986 Oct;125(1):130-40.
5
Studies of progressive glomerular sclerosis in the rat.大鼠进行性肾小球硬化的研究。
Am J Pathol. 1986 Jun;123(3):553-62.
6
Culture and characterization of bovine mesenteric lymphatic endothelium.
In Vitro Cell Dev Biol. 1987 Oct;23(10):698-706. doi: 10.1007/BF02620983.
7
8
Growth in vitro of cells from renal glomerulus. Difficulties in establishing long-term cultures of rat glomerular endothelium.
Int Urol Nephrol. 1989;21(1):105-12. doi: 10.1007/BF02549908.
9
Microvascular endothelial cell heterogeneity: interactions with leukocytes and tumor cells.微血管内皮细胞异质性：与白细胞和肿瘤细胞的相互作用
Cancer Metastasis Rev. 1990 Feb;8(4):353-89. doi: 10.1007/BF00052608.
本文引用的文献
1
NEW CYTOPLASMIC COMPONENTS IN ARTERIAL ENDOTHELIA.动脉内皮细胞中的新细胞质成分
J Cell Biol. 1964 Oct;23(1):101-12. doi: 10.1083/jcb.23.1.101.
2
Studies of morphologically atypical ("sprouting") cultures of bovine aortic endothelial cells. Growth characteristics and connective tissue protein synthesis.牛主动脉内皮细胞形态学非典型（“发芽”）培养物的研究。生长特性和结缔组织蛋白合成。
J Cell Physiol. 1980 Feb;102(2):183-91. doi: 10.1002/jcp.1041020209.
3
Phylogenetic analysis of platelet-derived growth factor by radio-receptor assay.通过放射受体分析法对血小板衍生生长因子进行系统发育分析。
J Cell Biol. 1982 Nov;95(2 Pt 1):667-71. doi: 10.1083/jcb.95.2.667.
4
Platelet-derived growth factor. I. High yield purification and evidence for multiple forms.血小板衍生生长因子。I. 高产率纯化及多种形式的证据。
J Biol Chem. 1982 May 10;257(9):5154-60.
5
Human glomerular cells in vitro: isolation and characterization.体外培养的人肾小球细胞：分离与鉴定。
Transplant Proc. 1980 Sep;12(3 Suppl 1):88-99.
6
Isolation and growth of endothelial cells from the microvessels of the newborn human foreskin in cell culture.在细胞培养中从新生儿包皮微血管分离内皮细胞并使其生长。
J Invest Dermatol. 1980 Oct;75(4):316-21. doi: 10.1111/1523-1747.ep12530941.
7
Human platelet-derived growth factor: radioimmunoassay and discovery of a specific plasma-binding protein.人血小板衍生生长因子：放射免疫测定及一种特异性血浆结合蛋白的发现。
J Cell Biol. 1983 Aug;97(2):383-8. doi: 10.1083/jcb.97.2.383.
8
Cellular outgrowth from isolated glomeruli. Origin and characterization.
Lab Invest. 1983 May;48(5):526-42.
9
Cultivation of microvascular endothelial cells from human preputial skin.
In Vitro. 1980 Aug;16(8):675-84. doi: 10.1007/BF02619197.
10
Expression of angiotensin-converting enzyme activity in cultured pulmonary artery endothelial cells.培养的肺动脉内皮细胞中血管紧张素转换酶活性的表达
J Cell Physiol. 1981 Sep;108(3):337-45. doi: 10.1002/jcp.1041080307.
Zotero 插件