使用聚阳离子和二甲基亚砜进行DNA转染的新方法。
New procedure for DNA transfection with polycation and dimethyl sulfoxide.
作者信息
Kawai S, Nishizawa M
出版信息
Mol Cell Biol. 1984 Jun;4(6):1172-4. doi: 10.1128/mcb.4.6.1172-1174.1984.
Abstract
A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl sulfoxide as an agent to facilitate the uptake of adsorbed DNA by the cells. In this new, simple, and convenient polycation-dimethyl sulfoxide transfection, which requires no carrier DNA even with small amounts of DNA, the number of transformed cell foci induced by Rous sarcoma virus DNA was proportional to the dose of the transfecting DNA, and chicken embryo fibroblast cells were successfully transformed by v-src-containing subgenomic DNA as well.
摘要
一种新的DNA转染方法已在鸡胚成纤维细胞系统和克隆的劳氏肉瘤病毒DNA中得以开发。该方法使用一种聚阳离子试剂作为介质，将DNA吸附到细胞表面，并用二甲基亚砜作为促进细胞摄取吸附DNA的试剂。在这种新型、简单且便捷的聚阳离子-二甲基亚砜转染方法中，即使使用少量DNA也无需载体DNA，劳氏肉瘤病毒DNA诱导的转化细胞集落数量与转染DNA的剂量成正比，并且含v-src的亚基因组DNA也成功地转化了鸡胚成纤维细胞。
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