Falkner F G, Zachau H G
Nature. 1984;310(5972):71-4. doi: 10.1038/310071a0.
Transcription of the immunoglobulin kappa light-chain genes depends on the presence of a TATA box upstream of the leader gene segment and is regulated by an enhancer sequence in the large intron. In studying a rearranged mouse kappa light-chain gene we have now found that sequences between--90 and--160 base pairs (bp) upstream of the coding region are essential for correct transcription in gene transfer experiments. This region contains the deca- and pentadecanucleotide sequences TNATTTGCAT and TGCAGCCTGTGNCCAG, which we call dc and pd, respectively. Sequences related to dc and pd were found upstream of all human and mouse kappa-chain variable region (Vk) genes, upstream of lambda-chain variable region (V lambda) genes, and within the mouse heavy-chain enhancer. An inverted and complementary form of the dc element (ATGCAAATNA, called cd) occurs upstream of all heavy-chain variable region (VH) genes. The newly defined sequences may be involved in the control of immunoglobulin gene transcription.
免疫球蛋白κ轻链基因的转录依赖于前导基因片段上游TATA框的存在，并受大内含子中一个增强子序列的调控。在研究一个重排的小鼠κ轻链基因时，我们现在发现，编码区上游-90至-160碱基对（bp）之间的序列对于基因转移实验中的正确转录至关重要。该区域包含十聚体和十五聚体序列TNATTTGCAT和TGCAGCCTGTGNCCAG，我们分别称之为dc和pd。在所有人类和小鼠κ链可变区（Vk）基因的上游、λ链可变区（Vλ）基因的上游以及小鼠重链增强子内都发现了与dc和pd相关的序列。dc元件的反向互补形式（ATGCAAATNA，称为cd）出现在所有重链可变区（VH）基因的上游。这些新定义的序列可能参与免疫球蛋白基因转录的调控。
