Frotscher M, Léránth C, Lübbers K, Oertel W H
Neurosci Lett. 1984 May 4;46(2):137-43. doi: 10.1016/0304-3940(84)90431-2.
The innervation of GABAergic hippocampal neurons by commissural fibers was investigated in the guinea pig by a combined anterograde degeneration - immunocytochemical technique. Presumed GABAergic neurons were identified by immunocytochemistry for glutamate decarboxylase (GAD) and the commissural fibers by electron-dense degeneration following contralateral transection of the fimbria. Commissural afferents were found to establish asymmetric synaptic contacts with non-pyramidal GAD-immunoreactive neurons located in subpyramidal and suprapyramidal zones of region CA1. The observed connection suggests that inhibition of pyramidal cells may occur in a feed-forward manner as postulated by electrophysiological studies.
采用顺行性变性-免疫细胞化学联合技术，在豚鼠中研究了连合纤维对海马GABA能神经元的神经支配。通过谷氨酸脱羧酶（GAD）免疫细胞化学鉴定假定的GABA能神经元，并在对侧切断海马伞后，通过电子致密变性鉴定连合纤维。发现连合传入纤维与位于CA1区锥体下和锥体上区域的非锥体GAD免疫反应性神经元建立不对称突触联系。观察到的这种联系表明，如电生理研究所假设的那样，锥体细胞的抑制可能以前馈方式发生。
