用4′-6-二脒基-2-苯基吲哚对姐妹染色单体进行差异荧光染色。
Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole.
作者信息
Lin M S, Alfi O S, Donnell G N
出版信息
Can J Genet Cytol. 1976 Sep;18(3):545-7. doi: 10.1139/g76-068.
DOI:10.1139/g76-068
PMID:63310
Abstract
Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.
摘要
在含有5-溴脱氧尿苷的培养基中生长两个复制周期的人淋巴细胞染色体中，姐妹染色单体的差异荧光和姐妹染色单体交换（SCE）在用4′-6-二脒基-2-苯基吲哚（DAPI）染色后，可通过荧光显微镜检测到。DAPI荧光似乎比染料33258 Hoechst的荧光更稳定，可能为检测SCE提供一种更灵敏的方法。
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