人脑肿瘤中乳酸脱氢酶同工酶的细胞化学变化

Cytochemical changes in lactate dehydrogenase isoenzymes in human brain tumours.

作者信息

Fujii M, Nishikawa A, Tanaka T, Mori H, Takahashi M, Sakai N, Yamada H

出版信息

Acta Neurochir (Wien). 1984;71(3-4):243-53. doi: 10.1007/BF01401319.

Abstract

The lactate dehydrogenase (LDH) isoenzyme patterns in benign and malignant brain tumours were determined by means of electrophoresis of the cell extracts and selective cytochemical stain of the smears. The LDH isoenzyme distribution of the cell extracts showed a pronounced cathodal shift in the malignant gliomas and metastatic carcinomas. Normal brain tissues and histologically benign gliomas, however, showed an anodal pattern with a dominance of the H-type LDH. Schwannomas and meningiomas had a midzone isoenzyme pattern with a dominant LDH3 fraction. Pituitary adenomas usually showed the LDH pattern similar to that of the normal cerebrum. The LDH M fraction could be cytochemically verified using an inhibitory effect by 2.6 M urea in staining. Astrocytomas grades 3-4 and metastatic carcinomas were characterized by loss or marked reduction of stainability by urea treatment, while astrocytomas grades 1-2 and oligodendrogliomas were resistant to urea inhibition.

摘要

通过细胞提取物的电泳和涂片的选择性细胞化学染色，测定了良性和恶性脑肿瘤中的乳酸脱氢酶（LDH）同工酶模式。细胞提取物的LDH同工酶分布在恶性胶质瘤和转移性癌中显示出明显的阴极迁移。然而，正常脑组织和组织学上良性的胶质瘤显示出阳极模式，以H型LDH为主。神经鞘瘤和脑膜瘤具有中区同工酶模式，以LDH3部分为主。垂体腺瘤通常显示出与正常大脑相似的LDH模式。可以通过2.6M尿素在染色中的抑制作用，对LDH M部分进行细胞化学验证。3-4级星形细胞瘤和转移性癌的特征是经尿素处理后染色性丧失或明显降低，而1-2级星形细胞瘤和少突胶质细胞瘤对尿素抑制有抗性。