Rabow L, Kristensson K
Acta Neurochir (Wien). 1977;36(1-2):71-81. doi: 10.1007/BF01405988.
The total activities of lactate dehydrogenase (LD) and the LD isoenzyme distribution were determined on homogenates from 61 benign and malignant intracranial tumours, and in serum and cerebrospinal fluid from 45 and 28 of the tumour patients respectively. The LD activity and the LD isoenzyme distribution in serum were not changed in patients with intracranial tumours. The LD activity in the cerebrospinal fluid was raised in about 50% of the tumour patients, but without any significant differences between patients with benign and malignant tumours. Except for very low activities in acoustic schwannomas and in pituitary adenomas, the tumour tissue did not differ significantly from normal brain in total LD activity. The LD isoenzyme distribution showed a pronounced cathodal shift in the grade 3-4 astrocytomas and the metastatic carcinomas, but an anodal shift in the grade 1-2 astrocytomas and the oligodendrogliomas. The meningiomas and the ocoustic schwannomas showed a midzone isoenzyme pattern with a dominant LD3 fraction. Knowledge of the LD isoenyme distribution can thus sometimes be a complement to the histological examination in the classification of brain tumours.
测定了61例良性和恶性颅内肿瘤匀浆以及分别来自45例和28例肿瘤患者血清及脑脊液中的乳酸脱氢酶（LD）总活性和LD同工酶分布。颅内肿瘤患者血清中的LD活性和LD同工酶分布没有变化。约50%的肿瘤患者脑脊液中的LD活性升高，但良性和恶性肿瘤患者之间无显著差异。除听神经瘤和垂体腺瘤中活性极低外，肿瘤组织的LD总活性与正常脑组织无显著差异。LD同工酶分布在3 - 4级星形细胞瘤和转移性癌中表现为明显的阴极移动，而在1 - 2级星形细胞瘤和少突胶质细胞瘤中表现为阳极移动。脑膜瘤和听神经瘤呈现中区同工酶模式，以LD3为主。因此，LD同工酶分布的知识有时可作为脑肿瘤分类中组织学检查的补充。
