O'Shea P S, Chappell J B
Biochem J. 1984 Apr 15;219(2):401-4. doi: 10.1042/bj2190401.
It is shown by titrating a suspension of rat liver mitochondria with either ADP or an uncoupler that a specific rate of respiration may not have a unique associated value of the protonmotive force. Alternatively, a specific protonmotive force may not be associated with a unique rate of respiration. It seems that the rate of respiration and the protonmotive force are more sensitive to the agents used for the titrations than to each other. Such observations are not easily explained by the chemiosmotic hypothesis. It is, however, possible provided that the proton conductivities, i.e. the rates of dissipation of the protonmotive force, are considered to be different for each of the agents used to titrate the rate of respiration at the same protonmotive force, or vice versa.
通过用ADP或解偶联剂滴定大鼠肝线粒体悬浮液表明，特定的呼吸速率可能没有唯一相关的质子动力势值。或者，特定的质子动力势可能与唯一的呼吸速率无关。似乎呼吸速率和质子动力势对用于滴定的试剂比对彼此更敏感。化学渗透假说不容易解释这些观察结果。然而，如果认为在相同质子动力势下用于滴定呼吸速率的每种试剂的质子电导率，即质子动力势的耗散速率是不同的，那么这就是可能的，反之亦然。
