线粒体电子传递过程中质子泄漏与氧化还原滑移的实验区分
Experimental discrimination between proton leak and redox slip during mitochondrial electron transport.
作者信息
Brand M D, Chien L F, Diolez P
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):27-9. doi: 10.1042/bj2970027.
Abstract
By measuring the relationship between protonmotive force and the increment in oxygen consumption by mitochondria treated with submaximal amounts of uncoupler, we have experimentally tested four different models of imperfect coupling of oxidative phosphorylation. The results show that the increased rate of oxygen consumption at high protonmotive force is explained entirely by the dependence on protonmotive force of the passive proton leak conductance of the mitochondrial inner membrane. There is no measurable contribution from redox-slip reactions in the proton pumps caused by high protonmotive force. Neither is there any contribution from increased proton conductance of the membrane or increased redox slip in the respiratory chain caused by high turnover rates of the complexes.
摘要
通过测量质子动力与用亚最大量解偶联剂处理的线粒体耗氧量增加之间的关系，我们通过实验测试了四种不同的氧化磷酸化不完全偶联模型。结果表明，高质子动力下耗氧率的增加完全是由线粒体内膜被动质子泄漏电导对质子动力的依赖性所解释的。高质子动力引起的质子泵中氧化还原滑移反应没有可测量的贡献。复合物高周转率引起的膜质子电导增加或呼吸链中氧化还原滑移增加也没有任何贡献。
相似文献
1
Experimental discrimination between proton leak and redox slip during mitochondrial electron transport.
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):27-9. doi: 10.1042/bj2970027.
2
The effect of chloroform on mitochondrial energy transduction.
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):837-45. doi: 10.1042/bj3200837.
3
The causes and functions of mitochondrial proton leak.
Biochim Biophys Acta. 1994 Aug 30;1187(2):132-9. doi: 10.1016/0005-2728(94)90099-x.
4
The apparent non-linearity of the relationship between the rate of respiration and the protonmotive force of mitochondria can be explained by heterogeneity of mitochondrial preparations.
FEBS Lett. 1985 Mar 25;182(2):243-8. doi: 10.1016/0014-5793(85)80307-0.
5
Mechanism of action of agents which uncouple oxidative phosphorylation: direct correlation between proton-carrying and respiratory-releasing properties using rat liver mitochondria.
Biochim Biophys Acta. 1975 May 15;387(2):234-40. doi: 10.1016/0005-2728(75)90106-1.
6
Quantitative analysis of some mechanisms affecting the yield of oxidative phosphorylation: dependence upon both fluxes and forces.
Mol Cell Biochem. 1998 Jul;184(1-2):35-52.
7
The relationship between the rate of respiration and the protonmotive force. The role of proton conductivity.
Biochem J. 1984 Apr 15;219(2):401-4. doi: 10.1042/bj2190401.
8
Oxidative phosphorylation in intact hepatocytes: quantitative characterization of the mechanisms of change in efficiency and cellular consequences.
Mol Cell Biochem. 1998 Jul;184(1-2):53-65.
9
Effect of protonmotive force on the relative proton stoichiometries of the mitochondrial proton pumps.
Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):75-80. doi: 10.1042/bj2750075.
10
Inhibition by butylmalonate of proton influx in nonphosphorylating mitochondria.
Arch Biochem Biophys. 1998 Jul 1;355(1):93-100. doi: 10.1006/abbi.1998.0709.
引用本文的文献
1
-hydroxybutyrate enhances brain metabolism in normoglycemia and hyperglycemia, providing cerebroprotection in a mouse stroke model.
J Cereb Blood Flow Metab. 2025 Apr 12:271678X251334222. doi: 10.1177/0271678X251334222.
2
The multiple facets of mitochondrial regulations controlling cellular thermogenesis.
Cell Mol Life Sci. 2022 Sep 20;79(10):525. doi: 10.1007/s00018-022-04523-8.
3
Prescription drugs and mitochondrial metabolism.
Biosci Rep. 2022 Apr 29;42(4). doi: 10.1042/BSR20211813.
4
A Comparative Study of the Action of Protonophore Uncouplers and Decoupling Agents as Inducers of Free Respiration in Mitochondria in States 3 and 4: Theoretical and Experimental Approaches.
Cell Biochem Biophys. 2020 Jun;78(2):203-216. doi: 10.1007/s12013-020-00914-5. Epub 2020 May 5.
5
An Equivocal Final Link - Quantitative Determination of the Thermodynamic Efficiency of ATP Hydrolysis - Sullies the Chain of Electric, Ionic, Mechanical and Metabolic Steps Underlying Cardiac Contraction.
Front Physiol. 2020 Mar 31;11:183. doi: 10.3389/fphys.2020.00183. eCollection 2020.
6
Use the Protonmotive Force: Mitochondrial Uncoupling and Reactive Oxygen Species.
J Mol Biol. 2018 Oct 19;430(21):3873-3891. doi: 10.1016/j.jmb.2018.03.025. Epub 2018 Apr 4.
7
The Bioenergetics of Exercise.
Cold Spring Harb Perspect Med. 2018 May 1;8(5):a029678. doi: 10.1101/cshperspect.a029678.
8
Hypothesis on Skeletal Muscle Aging: Mitochondrial Adenine Nucleotide Translocator Decreases Reactive Oxygen Species Production While Preserving Coupling Efficiency.
Front Physiol. 2015 Dec 16;6:369. doi: 10.3389/fphys.2015.00369. eCollection 2015.
9
Assessing mitochondrial dysfunction in cells.
Biochem J. 2011 Apr 15;435(2):297-312. doi: 10.1042/BJ20110162.
10
Accurate determination of the oxidative phosphorylation affinity for ADP in isolated mitochondria.
PLoS One. 2011;6(6):e20709. doi: 10.1371/journal.pone.0020709. Epub 2011 Jun 9.
本文引用的文献
1
Proton slippage in cytochrome c oxidase of Paracoccus denitrificans. Membrane-potential measurements with the two-subunit and three-subunit enzyme.
Eur J Biochem. 1993 Mar 15;212(3):827-31. doi: 10.1111/j.1432-1033.1993.tb17724.x.
2
Effect of funiculosin and antimycin A on the redox-driven H+-pumps in mitochondria: on the nature of "leaks'.
Eur J Biochem. 1981 Jul;117(2):389-94. doi: 10.1111/j.1432-1033.1981.tb06350.x.
3
Non-ohmic proton conductance of mitochondria and liposomes.
Biochemistry. 1984 Apr 10;23(8):1640-5. doi: 10.1021/bi00303a009.
4
Protonmotive functions of cytochrome c oxidase in reconstituted vesicles. Influence of turnover rate on 'proton translocation'.
Biochem J. 1983 Jan 15;210(1):199-205. doi: 10.1042/bj2100199.
5
Molecular slipping in redox and ATPase H+ pumps.
Biochim Biophys Acta. 1983 May 27;723(2):317-21. doi: 10.1016/0005-2728(83)90131-7.
6
The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution.
Eur J Biochem. 1974 Dec 16;50(1):305-15. doi: 10.1111/j.1432-1033.1974.tb03899.x.
7
Variable stoichiometry of proton pumping by the mitochondrial respiratory chain.
Nature. 1987;329(6135):170-2. doi: 10.1038/329170a0.
8
Changes in permeability to protons and other cations at high proton motive force in rat liver mitochondria.
Biochem J. 1986 Feb 15;234(1):75-81. doi: 10.1042/bj2340075.
9
Uncoupling of oxidative phosphorylation. 2. Alternative mechanisms: intrinsic uncoupling or decoupling?
Biochemistry. 1987 Nov 17;26(23):7339-47. doi: 10.1021/bi00397a022.
10
Intrinsic uncoupling of mitochondrial proton pumps. 1. Non-ohmic conductance cannot account for the nonlinear dependence of static head respiration on delta microH.
Biochemistry. 1986 Feb 25;25(4):760-7. doi: 10.1021/bi00352a004.
Zotero 插件