线粒体电子传递过程中质子泄漏与氧化还原滑移的实验区分
Experimental discrimination between proton leak and redox slip during mitochondrial electron transport.
作者信息
Brand M D, Chien L F, Diolez P
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):27-9. doi: 10.1042/bj2970027.
Abstract
By measuring the relationship between protonmotive force and the increment in oxygen consumption by mitochondria treated with submaximal amounts of uncoupler, we have experimentally tested four different models of imperfect coupling of oxidative phosphorylation. The results show that the increased rate of oxygen consumption at high protonmotive force is explained entirely by the dependence on protonmotive force of the passive proton leak conductance of the mitochondrial inner membrane. There is no measurable contribution from redox-slip reactions in the proton pumps caused by high protonmotive force. Neither is there any contribution from increased proton conductance of the membrane or increased redox slip in the respiratory chain caused by high turnover rates of the complexes.
摘要
通过测量质子动力与用亚最大量解偶联剂处理的线粒体耗氧量增加之间的关系，我们通过实验测试了四种不同的氧化磷酸化不完全偶联模型。结果表明，高质子动力下耗氧率的增加完全是由线粒体内膜被动质子泄漏电导对质子动力的依赖性所解释的。高质子动力引起的质子泵中氧化还原滑移反应没有可测量的贡献。复合物高周转率引起的膜质子电导增加或呼吸链中氧化还原滑移增加也没有任何贡献。
相似文献
1
Experimental discrimination between proton leak and redox slip during mitochondrial electron transport.线粒体电子传递过程中质子泄漏与氧化还原滑移的实验区分
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):27-9. doi: 10.1042/bj2970027.
2
The effect of chloroform on mitochondrial energy transduction.氯仿对线粒体能量转导的影响。
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):837-45. doi: 10.1042/bj3200837.
3
The causes and functions of mitochondrial proton leak.线粒体质子泄漏的原因及功能。
Biochim Biophys Acta. 1994 Aug 30;1187(2):132-9. doi: 10.1016/0005-2728(94)90099-x.
4
The apparent non-linearity of the relationship between the rate of respiration and the protonmotive force of mitochondria can be explained by heterogeneity of mitochondrial preparations.
FEBS Lett. 1985 Mar 25;182(2):243-8. doi: 10.1016/0014-5793(85)80307-0.
5
Mechanism of action of agents which uncouple oxidative phosphorylation: direct correlation between proton-carrying and respiratory-releasing properties using rat liver mitochondria.使氧化磷酸化解偶联的药物的作用机制：利用大鼠肝脏线粒体对质子携带特性与呼吸释放特性之间的直接关联
Biochim Biophys Acta. 1975 May 15;387(2):234-40. doi: 10.1016/0005-2728(75)90106-1.
6
Quantitative analysis of some mechanisms affecting the yield of oxidative phosphorylation: dependence upon both fluxes and forces.影响氧化磷酸化产率的一些机制的定量分析：对通量和力的依赖性。
Mol Cell Biochem. 1998 Jul;184(1-2):35-52.
7
The relationship between the rate of respiration and the protonmotive force. The role of proton conductivity.呼吸速率与质子动力之间的关系。质子传导性的作用。
Biochem J. 1984 Apr 15;219(2):401-4. doi: 10.1042/bj2190401.
8
9
Effect of protonmotive force on the relative proton stoichiometries of the mitochondrial proton pumps.质子动力对线粒体质子泵相对质子化学计量的影响。
Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):75-80. doi: 10.1042/bj2750075.
10
Inhibition by butylmalonate of proton influx in nonphosphorylating mitochondria.丁基丙二酸对非磷酸化线粒体中质子内流的抑制作用。
Arch Biochem Biophys. 1998 Jul 1;355(1):93-100. doi: 10.1006/abbi.1998.0709.
引用本文的文献
1
-hydroxybutyrate enhances brain metabolism in normoglycemia and hyperglycemia, providing cerebroprotection in a mouse stroke model.β-羟基丁酸可增强正常血糖和高血糖状态下的脑代谢，在小鼠中风模型中提供脑保护作用。
J Cereb Blood Flow Metab. 2025 Apr 12:271678X251334222. doi: 10.1177/0271678X251334222.
2
The multiple facets of mitochondrial regulations controlling cellular thermogenesis.控制细胞产热的线粒体调节的多个方面。
Cell Mol Life Sci. 2022 Sep 20;79(10):525. doi: 10.1007/s00018-022-04523-8.
3
Prescription drugs and mitochondrial metabolism.处方药与线粒体代谢
Biosci Rep. 2022 Apr 29;42(4). doi: 10.1042/BSR20211813.
4
A Comparative Study of the Action of Protonophore Uncouplers and Decoupling Agents as Inducers of Free Respiration in Mitochondria in States 3 and 4: Theoretical and Experimental Approaches.质子载体解偶联剂和去偶剂在诱导 3 态和 4 态线粒体自由呼吸方面的作用比较研究：理论和实验方法。
Cell Biochem Biophys. 2020 Jun;78(2):203-216. doi: 10.1007/s12013-020-00914-5. Epub 2020 May 5.
5
An Equivocal Final Link - Quantitative Determination of the Thermodynamic Efficiency of ATP Hydrolysis - Sullies the Chain of Electric, Ionic, Mechanical and Metabolic Steps Underlying Cardiac Contraction.一个模棱两可的最终环节——ATP水解热力学效率的定量测定——破坏了心脏收缩背后的电、离子、机械和代谢步骤的链条。
Front Physiol. 2020 Mar 31;11:183. doi: 10.3389/fphys.2020.00183. eCollection 2020.
6
Use the Protonmotive Force: Mitochondrial Uncoupling and Reactive Oxygen Species.利用质子动力：线粒体解偶联和活性氧物质。
J Mol Biol. 2018 Oct 19;430(21):3873-3891. doi: 10.1016/j.jmb.2018.03.025. Epub 2018 Apr 4.
7
The Bioenergetics of Exercise.运动的生物能量学。
Cold Spring Harb Perspect Med. 2018 May 1;8(5):a029678. doi: 10.1101/cshperspect.a029678.
8
Hypothesis on Skeletal Muscle Aging: Mitochondrial Adenine Nucleotide Translocator Decreases Reactive Oxygen Species Production While Preserving Coupling Efficiency.骨骼肌衰老假说：线粒体腺嘌呤核苷酸转运体在保持偶联效率的同时降低活性氧生成。
Front Physiol. 2015 Dec 16;6:369. doi: 10.3389/fphys.2015.00369. eCollection 2015.
9
Assessing mitochondrial dysfunction in cells.评估细胞中的线粒体功能障碍。
Biochem J. 2011 Apr 15;435(2):297-312. doi: 10.1042/BJ20110162.
10
Accurate determination of the oxidative phosphorylation affinity for ADP in isolated mitochondria.准确测定分离线粒体中 ADP 的氧化磷酸化亲和力。
PLoS One. 2011;6(6):e20709. doi: 10.1371/journal.pone.0020709. Epub 2011 Jun 9.
本文引用的文献
1
Proton slippage in cytochrome c oxidase of Paracoccus denitrificans. Membrane-potential measurements with the two-subunit and three-subunit enzyme.反硝化副球菌细胞色素c氧化酶中的质子泄漏。对两亚基和三亚基酶的膜电位测量。
Eur J Biochem. 1993 Mar 15;212(3):827-31. doi: 10.1111/j.1432-1033.1993.tb17724.x.
2
Effect of funiculosin and antimycin A on the redox-driven H+-pumps in mitochondria: on the nature of "leaks'.真菌毒素和抗霉素A对线粒体中氧化还原驱动的H⁺泵的影响：关于“泄漏”的本质
Eur J Biochem. 1981 Jul;117(2):389-94. doi: 10.1111/j.1432-1033.1981.tb06350.x.
3
Non-ohmic proton conductance of mitochondria and liposomes.线粒体和脂质体的非欧姆质子传导
Biochemistry. 1984 Apr 10;23(8):1640-5. doi: 10.1021/bi00303a009.
4
Protonmotive functions of cytochrome c oxidase in reconstituted vesicles. Influence of turnover rate on 'proton translocation'.重组囊泡中细胞色素c氧化酶的质子动力功能。周转率对“质子转运”的影响。
Biochem J. 1983 Jan 15;210(1):199-205. doi: 10.1042/bj2100199.
5
Molecular slipping in redox and ATPase H+ pumps.氧化还原与ATP酶H⁺泵中的分子滑动
Biochim Biophys Acta. 1983 May 27;723(2):317-21. doi: 10.1016/0005-2728(83)90131-7.
6
The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by ion distribution.通过离子分布测定呼吸作用和ATP水解对大鼠肝脏线粒体内膜质子电化学梯度的影响。
Eur J Biochem. 1974 Dec 16;50(1):305-15. doi: 10.1111/j.1432-1033.1974.tb03899.x.
7
Variable stoichiometry of proton pumping by the mitochondrial respiratory chain.
Nature. 1987;329(6135):170-2. doi: 10.1038/329170a0.
8
Changes in permeability to protons and other cations at high proton motive force in rat liver mitochondria.大鼠肝线粒体在高质子动力势下对质子和其他阳离子通透性的变化
Biochem J. 1986 Feb 15;234(1):75-81. doi: 10.1042/bj2340075.
9
Uncoupling of oxidative phosphorylation. 2. Alternative mechanisms: intrinsic uncoupling or decoupling?
Biochemistry. 1987 Nov 17;26(23):7339-47. doi: 10.1021/bi00397a022.
10
Intrinsic uncoupling of mitochondrial proton pumps. 1. Non-ohmic conductance cannot account for the nonlinear dependence of static head respiration on delta microH.
Biochemistry. 1986 Feb 25;25(4):760-7. doi: 10.1021/bi00352a004.
Zotero 插件