Brand M D, Chien L F, Diolez P
Department of Biochemistry, University of Cambridge, U.K.
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):27-9. doi: 10.1042/bj2970027.
By measuring the relationship between protonmotive force and the increment in oxygen consumption by mitochondria treated with submaximal amounts of uncoupler, we have experimentally tested four different models of imperfect coupling of oxidative phosphorylation. The results show that the increased rate of oxygen consumption at high protonmotive force is explained entirely by the dependence on protonmotive force of the passive proton leak conductance of the mitochondrial inner membrane. There is no measurable contribution from redox-slip reactions in the proton pumps caused by high protonmotive force. Neither is there any contribution from increased proton conductance of the membrane or increased redox slip in the respiratory chain caused by high turnover rates of the complexes.
通过测量质子动力与用亚最大量解偶联剂处理的线粒体耗氧量增加之间的关系，我们通过实验测试了四种不同的氧化磷酸化不完全偶联模型。结果表明，高质子动力下耗氧率的增加完全是由线粒体内膜被动质子泄漏电导对质子动力的依赖性所解释的。高质子动力引起的质子泵中氧化还原滑移反应没有可测量的贡献。复合物高周转率引起的膜质子电导增加或呼吸链中氧化还原滑移增加也没有任何贡献。
