Rich A M, Weissmann G, Anderson C, Vosshall L, Haines K A, Humphreys T, Dunham P
Biochem Biophys Res Commun. 1984 Jun 29;121(3):863-70. doi: 10.1016/0006-291x(84)90757-5.
Dissociated cells of the marine sponge, Microciona prolifera, aggregate in response to a species-specific aggregation factor (MAF) and Ca ionophores. We now report that leukotriene B4, a 5-lipoxygenase product, also causes aggregation of sponge cells. No other lipoxygenase products provoked aggregation. However, nordihydroguaiaretic acid (NDGA), colchicine, indomethacin, piroxicam and ibuprofen inhibited MAF-induced aggregation; the latter three agents inhibited Ca movements. Inhibition of prostaglandin release cannot be the mechanism whereby these agents inhibit aggregation of sponge cells, since the cells do not respond to exogenous cyclooxygenase products.
海洋海绵动物微芒海绵（Microciona prolifera）的离体细胞会对物种特异性聚集因子（MAF）和钙离子载体产生聚集反应。我们现在报告，5-脂氧合酶产物白三烯B4也会导致海绵细胞聚集。没有其他脂氧合酶产物引发聚集。然而，去甲二氢愈创木酸（NDGA）、秋水仙碱、吲哚美辛、吡罗昔康和布洛芬抑制MAF诱导的聚集；后三种药物抑制钙离子移动。抑制前列腺素释放不可能是这些药物抑制海绵细胞聚集的机制，因为细胞对外源性环氧化酶产物没有反应。
