Wantzin G L, Larsen J K, Christensen I J, Ralfkiaer E, Thomsen K
Cancer. 1984 Oct 1;54(7):1348-52. doi: 10.1002/1097-0142(19841001)54:7<1348::aid-cncr2820540719>3.0.co;2-4.
Seven patients with mycosis fungoides early plaque stage with nondiagnostic histology had single-cell DNA content measured by flow cytometry for estimation of clonal ploidy. A total of 63 skin specimens were examined by histology and DNA measurements concurrently during the course of the disease. In addition, six patients had blood samples studied. All seven patients demonstrated aneuploid DNA histograms when the specimens were obtained from skin lesions. In 36 specimens the aneuploid peaks were hyperdiploid. By sequential studies one patient demonstrated two different aneuploid cell clones, one located in the hyperdiploid region and one located in the hypotetraploid region. All patients developed mycosis fungoides which were histologically confirmed, and the time from first aneuploid DNA histogram until diagnostic histology varied from 5 to 21 months (median, 12 months). In six of the patients a normal diploid DNA histogram was found of peripheral blood lymphocytes. The finding of aneuploidy in patients with early mycosis fungoides who still have a nondiagnostic histology emphasizes the value of flow cytometry as a complementary diagnostic aid which facilitates an early diagnosis.
7例蕈样肉芽肿早期斑块期且组织学检查无法确诊的患者，通过流式细胞术测量单细胞DNA含量以评估克隆倍体性。在疾病过程中，共对63个皮肤标本同时进行了组织学和DNA测量。此外，对6例患者的血液样本进行了研究。当从皮肤病变获取标本时，所有7例患者均表现出非整倍体DNA直方图。在36个标本中，非整倍体峰为超二倍体。通过连续研究，1例患者显示出两个不同的非整倍体细胞克隆，一个位于超二倍体区域，一个位于亚四倍体区域。所有患者均发展为经组织学证实的蕈样肉芽肿，从首次出现非整倍体DNA直方图到确诊组织学的时间为5至21个月（中位数为12个月）。6例患者外周血淋巴细胞的DNA直方图为正常二倍体。在组织学仍无法确诊的蕈样肉芽肿早期患者中发现非整倍体，强调了流式细胞术作为一种辅助诊断手段的价值，有助于早期诊断。
