Hagedorn M, Kiefer G
Arch Dermatol Res (1975). 1977 Apr 27;258(2):127-34. doi: 10.1007/BF00561617.
DNA content of mycosis infiltrate cells was measured in 5 patients with the Feulgen cytophotometric method in the plaque and tumor stages. In addition, the infiltrate cells were differentiated cytochemically into histiocytes and atypical lymphoid cells with NaF-sensitive naphthol-AS-D-acetate esterase. In no case was an aneuploid stem line demonstrated. However, increasing duration of the tumor stage was associated with a larger proportion of tetraploid and octoploid cells. The DNA histograms also exhibited a local proliferation of atypical lymphoid cells. This proliferation was arrested by cytostatic therapy. Comparison with semi-thin-sections of tumor tissue showed that the mycosis fungoides cells are atypical lymphoid cells. These DNA measurements do not contradict the concept of limited aneuploidy, as reported in cytogenic studies. Thus mycosis fungoides fits in with the DNA distribution pattern in the group of lymphomas.
采用福尔根细胞光度法对5例处于斑块期和肿瘤期的蕈样肉芽肿患者浸润细胞的DNA含量进行了测定。此外，利用对氟化钠敏感的萘酚-AS-D-醋酸酯酶，通过细胞化学方法将浸润细胞区分为组织细胞和非典型淋巴细胞。未发现非整倍体干细胞系。然而，肿瘤期持续时间的延长与四倍体和八倍体细胞比例的增加有关。DNA直方图还显示非典型淋巴细胞有局部增殖。这种增殖可被细胞抑制疗法阻止。与肿瘤组织半薄切片的比较表明，蕈样肉芽肿细胞是非典型淋巴细胞。这些DNA测量结果与细胞遗传学研究中报道的有限非整倍体概念并不矛盾。因此，蕈样肉芽肿符合淋巴瘤组中的DNA分布模式。
