Weitlauf H M
J Reprod Fertil. 1978 Mar;52(2):321-5. doi: 10.1530/jrf.0.0520321.
Fluid flushed from the uteri of "delayed implanting" and "implanting" mice was fractionated by gel-filtration. The fractions were freeze-dried, individually resuspended in culture medium containing [3H]uridine, and used for incubating "implanting" blastocysts. Several factors were found that reduced incorporation of [3H]uridine by the blastocysts. The amount of inhibitory activity in corresponding factors was generally similar in flushings from both types of uteri, but there was significantly more inhibitory activity in the void volume fractions of flushings from "delayed implanting" animals and it is suggested that this factor may be responsible for the metabolic dormancy of embryos during the diapause associated with delayed implantation.
从“延迟着床”和“正在着床”小鼠子宫中冲洗出的液体通过凝胶过滤进行分级分离。各分级分离物经冷冻干燥后，分别重悬于含有[³H]尿苷的培养基中，并用于培养“正在着床”的囊胚。发现了几种能降低囊胚对[³H]尿苷摄取的因子。相应因子中的抑制活性量在两种子宫冲洗液中通常相似，但“延迟着床”动物冲洗液的空体积分级分离物中的抑制活性明显更多，有人认为该因子可能是与延迟着床相关的滞育期间胚胎代谢休眠的原因。
