Reddy M M, Chinoy P, Grieco M H
J Biol Response Mod. 1984 Aug;3(4):379-86.
Natural Killer (NK) cell activity of peripheral blood mononuclear cells (PBMC) against K562 myeloid cells was studied in six normal heterosexual men and six patients with acquired immune deficiency syndrome (AIDS). The mean NK cell activity in six patients with AIDS was 28.74 +/- 6.82% (SEM) compared with 47.24 +/- 7.26% in six normal heterosexual men (p less than 0.10). PBMC obtained from these subjects were incubated overnight in complete medium alone, complete medium supplemented with interferon-alpha 2 (IFN-alpha 2), complete medium supplemented with interleukin-2 (IL-2), and complete medium supplemented with both IFN-alpha 2 and IL-2. Incubation of PBMC with IFN-alpha 2 enhanced the NK cell activity significantly (73.07 +/- 4.22%, p less than 0.025) in normal heterosexual men, but not in patients with AIDS (38.22 +/- 7.10%, p greater than 0.05). In contrast, IL-2 significantly enhanced NK cell activity in both patients with AIDS (57.16 +/- 8.4%, p less than 0.05) and normal heterosexual men (71.58 +/- 5.87%, p less than 0.05). When PBMC from both groups of subjects were incubated with both IFN-alpha 2 and IL-2, the NK cell activity increased to levels higher than those seen with either IFN-alpha 2 or IL-2 alone. These results suggest that IL-2 is capable of augmenting in vitro NK cell-mediated cytotoxicity with cells from AIDS patients.
在6名正常异性恋男性和6名获得性免疫缺陷综合征（艾滋病）患者中，研究了外周血单个核细胞（PBMC）对K562髓细胞的自然杀伤（NK）细胞活性。6名艾滋病患者的平均NK细胞活性为28.74±6.82%（标准误），而6名正常异性恋男性为47.24±7.26%（p<0.10）。从这些受试者获得的PBMC分别在单独的完全培养基、补充了干扰素-α2（IFN-α2）的完全培养基、补充了白细胞介素-2（IL-2）的完全培养基以及同时补充了IFN-α2和IL-2的完全培养基中过夜培养。在正常异性恋男性中，PBMC与IFN-α2孵育可显著增强NK细胞活性（73.07±4.22%，p<0.025），但在艾滋病患者中则不然（38.22±7.10%，p>0.05）。相反，IL-2在艾滋病患者（57.16±8.4%，p<0.05）和正常异性恋男性（71.58±5.87%，p<0.05）中均显著增强了NK细胞活性。当两组受试者的PBMC与IFN-α2和IL-2同时孵育时，NK细胞活性增加到高于单独使用IFN-α2或IL-2时的水平。这些结果表明，IL-2能够增强艾滋病患者细胞的体外NK细胞介导的细胞毒性。
