Valyakina T I, Vershinina N V, Gilyarevskii S D, Nesmeyanov V A
Mol Immunol. 1984 Sep;21(9):811-4. doi: 10.1016/0161-5890(84)90168-8.
The production of interleukin 2 by rat splenocytes, stimulated with Con A in serum-free medium, was studied. Under optimal conditions the stimulation in serum-containing and serum-free media resulted in identical interleukin 2 titers. The addition of phorbol myristate acetate prior or simultaneously with Con A did not augment interleukin 2 production. In contrast, preincubation of lymphocytes in serum-free medium for 24-36 hrs brought about a 8-10-fold increase of interleukin 2 titer. The interleukin 2 titer 4 hrs after the induction of preincubated lymphocytes with Con A was about 20% of maximal activity. The possible mechanism of early interleukin 2 production is under discussion.
研究了在无血清培养基中用刀豆蛋白A刺激大鼠脾细胞产生白细胞介素2的情况。在最佳条件下，含血清和无血清培养基中的刺激产生了相同的白细胞介素2效价。在刀豆蛋白A之前或同时添加佛波醇肉豆蔻酸酯不会增加白细胞介素2的产生。相反，淋巴细胞在无血清培养基中预孵育24 - 36小时会使白细胞介素2效价增加8 - 10倍。用刀豆蛋白A诱导预孵育淋巴细胞4小时后的白细胞介素2效价约为最大活性的20%。早期白细胞介素2产生的可能机制正在讨论中。
