经MLA 144细胞条件培养的培养基中白细胞介素-2产量增加。

Increased yields of IL-2 in media conditioned by MLA 144 cells.

作者信息

Aspinall R, O'Gorman A

出版信息

J Immunol Methods. 1987 Jul 16;101(1):79-84. doi: 10.1016/0022-1759(87)90219-5.

Abstract

The gibbon leukaemia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 X 10(5) cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 X 10(-5) M and PMA at 10 ng/ml.

摘要

长臂猿白血病细胞系MLA 144组成性地产生白细胞介素-2（IL-2），并且已被用作从一系列物种中获取IL-2以用于T细胞体外生长的来源。我们试图通过控制生长条件并使用已知能增加其他细胞系中IL-2产生的化合物，来增加该细胞系产生的条件培养基中IL-2的含量。我们发现，从最初以4×10⁵个细胞/毫升接种于含10%胎牛血清（FBS）、补充有5×10⁻⁵ M 2-巯基乙醇（2-ME）和10纳克/毫升佛波酯（PMA）的杜氏改良 Eagle培养基（DMEM）中的MLA 144细胞的72小时培养物中，可以获得含有更高含量IL-2的条件培养基。