Efrat S, Zelig S, Yagen B, Kaempfer R
Biochem Biophys Res Commun. 1984 Sep 17;123(2):842-8. doi: 10.1016/0006-291x(84)90307-3.
Stimulation of human lymphocytes with phytohemagglutinin results in the induction of messenger RNA encoding interleukin-2 (IL-2), a lymphokine possessing immuno-regulatory properties. We have previously demonstrated a dramatic superinduction of the formation of IL-2 and of biologically active IL-2 mRNA in the presence of cycloheximide, an inhibitor of translation. These findings suggested the involvement of a labile protein repressor in the regulation of human IL-2 gene expression. Here, we show a commensurate superinduction of IL-2 mRNA sequences by three additional inhibitors of protein synthesis with distinct modes of action: T-2 toxin, pactamycin, and sparsomycin. These results strengthen the concept that a labile protein controls the formation of mature IL-2 mRNA. They tend to eliminate the possibility that the superinduction phenomenon observed in the presence of cycloheximide is due to its action on a process other than translation.
用植物血凝素刺激人淋巴细胞会导致编码白细胞介素-2（IL-2）的信使核糖核酸的诱导，IL-2是一种具有免疫调节特性的淋巴因子。我们之前已经证明，在翻译抑制剂环己酰亚胺存在的情况下，IL-2的形成以及具有生物活性的IL-2信使核糖核酸会出现显著的超诱导现象。这些发现表明，一种不稳定的蛋白质阻遏物参与了人类IL-2基因表达的调控。在此，我们展示了另外三种具有不同作用方式的蛋白质合成抑制剂：T-2毒素、密旋霉素和稀疏霉素，对IL-2信使核糖核酸序列产生了相应的超诱导作用。这些结果强化了一种不稳定蛋白质控制成熟IL-2信使核糖核酸形成的概念。它们倾向于排除在环己酰亚胺存在下观察到的超诱导现象是由于其对翻译以外的过程产生作用的可能性。
