Siggens K W, Morris A G
Biochem Biophys Res Commun. 1985 Nov 27;133(1):274-9. doi: 10.1016/0006-291x(85)91871-6.
Human peripheral blood mononuclear lymphocytes produce interferon gamma (IFN-gamma) in response to stimulation by mitogens. Previous studies on the kinetics of IFN-gamma mRNA production upon mitogen induction, showed that steady-state levels of mRNA increased to a maximum at 12-24 h post-induction after which they declined to levels not detectable by the assay used. We show here that in mitogen induced peripheral blood lymphocytes, inhibition of protein synthesis using three different inhibitors (cycloheximide, puromycin, pactamycin) resulted in an increase in the steady-state levels of IFN-gamma mRNA. The levels of mRNA in cells treated with inhibitor 16 h post-induction were up to 3-fold higher than in untreated cells. Superinduction was possible up to 40 h post-induction after which the steady-state levels of mRNA had declined to limits below detection; IFN-gamma mRNA was not superinduced by cycloheximide in the presence of actinomycin D.
人外周血单个核淋巴细胞在有丝分裂原刺激下产生γ干扰素（IFN-γ）。先前关于有丝分裂原诱导后IFN-γ mRNA产生动力学的研究表明，mRNA的稳态水平在诱导后12 - 24小时增加到最大值，之后下降到所用检测方法无法检测到的水平。我们在此表明，在有丝分裂原诱导的外周血淋巴细胞中，使用三种不同的抑制剂（环己酰亚胺、嘌呤霉素、密旋霉素）抑制蛋白质合成会导致IFN-γ mRNA的稳态水平增加。诱导后16小时用抑制剂处理的细胞中mRNA水平比未处理细胞高3倍。超诱导可持续到诱导后40小时，之后mRNA的稳态水平下降到检测限以下；在放线菌素D存在的情况下，环己酰亚胺不会使IFN-γ mRNA超诱导。
