Baryshnikov A Iu
Biull Eksp Biol Med. 1984 Sep;98(9):324-6.
Hybridomas producing monoclonal antibodies against cortical thymocytes (IKO-11) and early T cell precursors (IKO-10) were obtained. Monoclonal IKO-11 antibodies reacted with 75 +/- 1.6% of thymocytes and IKO-10 with 2.5 +/- 0.5%. Thymocyte fractionation over 19-33% gradient showed IKO-10 antibodies to react with the first fraction containing large cells and IKO-11 antibodies with the 4th-6th fraction containing small cells. The antibodies did not react with peripheral blood cells from healthy donors but detected antigens in patients with T cell ALL. If IKO-10 antibodies reacted with T cell ALL, there was no IKO-11 antibody reaction and vice versa. IKO-10 antibodies recognized this antigen on the cell surface of T cell Molt-4, SKCO-3 and non-T, non-B ALL Reh-6 lines. IKO antibodies did not react with these cells.
获得了产生抗皮质胸腺细胞单克隆抗体（IKO - 11）和抗早期T细胞前体单克隆抗体（IKO - 10）的杂交瘤。单克隆IKO - 11抗体与75±1.6%的胸腺细胞发生反应，而IKO - 10抗体与2.5±0.5%的胸腺细胞发生反应。在19 - 33%的梯度上对胸腺细胞进行分级分离显示，IKO - 10抗体与含有大细胞的第一级分发生反应，而IKO - 11抗体与含有小细胞的第4 - 6级分发生反应。这些抗体不与健康供体的外周血细胞发生反应，但能在T细胞急性淋巴细胞白血病患者中检测到抗原。如果IKO - 10抗体与T细胞急性淋巴细胞白血病发生反应，则不存在IKO - 11抗体反应，反之亦然。IKO - 10抗体在T细胞Molt - 4、SKCO - 3以及非T、非B急性淋巴细胞白血病Reh - 6细胞系的细胞表面识别该抗原。IKO抗体不与这些细胞发生反应。
