Nalet V, Fournier C
Cell Immunol. 1984 Nov;89(1):212-22. doi: 10.1016/0008-8749(84)90211-9.
The relationship between autorosettes and allorosettes was investigated using a mixed rosette assay in which the origin of the erythrocytes was assessed by the fluorescein isothiocyanate (FITC) labeling of one type of erythrocyte. The data show that auto- and allorosettes belong to the same T-cell subset: in most of the subjects, the percentages of T cells binding autologous red blood cells (auto-RBC) are equivalent to those binding allogeneic RBC (allo-RBC); the percentage of rosettes formed after the simultaneous addition of auto- and allo-RBC is similar to that of autorosettes alone or allorosettes alone; and nearly 80% of the rosetting cells bind both types of RBC as directly visualized in the mixed rosette assay. The experiments in which the lymphocytes are rosetted first with one type of RBC, and then with the other type support the finding that auto- and allo-RBC may bind to the lymphocytes through a single receptor which exhibits a varying affinity for RBC according to their origin.
利用混合玫瑰花结试验研究自身玫瑰花结与同种异体玫瑰花结之间的关系，在该试验中，通过对一种红细胞进行异硫氰酸荧光素（FITC）标记来评估红细胞的来源。数据表明，自身玫瑰花结和同种异体玫瑰花结属于同一T细胞亚群：在大多数受试者中，与自身红细胞（auto-RBC）结合的T细胞百分比与与同种异体红细胞（allo-RBC）结合的T细胞百分比相当；同时加入自身红细胞和同种异体红细胞后形成的玫瑰花结百分比与单独的自身玫瑰花结或单独的同种异体玫瑰花结相似；并且在混合玫瑰花结试验中直接观察到，近80%的玫瑰花结形成细胞与两种类型的红细胞都结合。先让淋巴细胞与一种红细胞形成玫瑰花结，然后再与另一种红细胞形成玫瑰花结的实验支持了这一发现，即自身红细胞和同种异体红细胞可能通过单一受体与淋巴细胞结合，该受体根据红细胞的来源对其表现出不同的亲和力。
