一种通过与自体红细胞形成玫瑰花结来检测和分离T淋巴细胞的简单快速方法。

A simple and rapid method for detecting and separating T lymphocytes by rosetting with autologous erythrocytes.

作者信息

Volk H D, Grunow R, Neuhaus K, Effenberger E

出版信息

J Immunol Methods. 1984 Oct 26;73(2):443-6. doi: 10.1016/0022-1759(84)90419-8.

Abstract

Counting and separation of auto-rosette-forming lymphocytes is a critical procedure since autologous rosettes are very unstable. It was demonstrated that human serum albumin efficiently stabilizes autologous rosettes, and makes it possible to distinguish at least three T-cell subsets by their different affinities for autologous erythrocytes. About 1% of T-cells have a high affinity for autologous erythrocytes and about 30% intermediate affinity. With a careful procedure, it is possible to detect a further subpopulation with very low affinity for erythrocytes. The sum of these three subsets was about 75% of the T-cells in the peripheral blood.

摘要

自体玫瑰花结形成淋巴细胞的计数和分离是一个关键步骤，因为自体玫瑰花结非常不稳定。已证明人血清白蛋白能有效稳定自体玫瑰花结，并使得通过它们对自体红细胞的不同亲和力区分至少三个T细胞亚群成为可能。约1%的T细胞对自体红细胞具有高亲和力，约30%具有中等亲和力。通过仔细操作，有可能检测到对红细胞亲和力极低的另一个亚群。这三个亚群的总和约占外周血中T细胞的75%。