Søndergaard I, Weeke B
Allergy. 1984 Aug;39(6):473-9. doi: 10.1111/j.1398-9995.1984.tb01971.x.
A method is described for bioaffinity chromatographic purification of patient-specific IgE reactive allergens by using sepharose coupled rabbit anti-human IgE antibodies, patient serum and an allergen extract. The conditions for this bioaffinity chromatographic procedure have been optimised, and it was shown that the nonspecific binding of serum proteins and antigens can be kept at very low levels by a high ionic strength. The specificity of the method has been determined by showing that allergens to which the patient did not react were not bound to the affinity matrix. We suggest that the isolated allergens could be used for more specific immunotherapy in the future.
描述了一种通过使用偶联琼脂糖的兔抗人IgE抗体、患者血清和变应原提取物对患者特异性IgE反应性变应原进行生物亲和色谱纯化的方法。该生物亲和色谱程序的条件已得到优化，结果表明，通过高离子强度可将血清蛋白和抗原的非特异性结合保持在非常低的水平。通过证明患者无反应的变应原不与亲和基质结合，确定了该方法的特异性。我们建议，分离出的变应原将来可用于更特异性的免疫疗法。
