Yonei S, Akasaka S, Kato M
Int J Radiat Biol Relat Stud Phys Chem Med. 1984 Oct;46(4):463-71. doi: 10.1080/09553008414551671.
The structural changes of human erythrocyte membranes after X-irradiation were investigated with the aid of fluorescent probes. It was found that the fluorescence characteristics (intensity, polarization and the dissociation constant) of 1-anilino-8-naphthalene sulphonate (ANS) bound to X-irradiated (up to 40 Gy) membranes were quite different from those in unirradiated ones. Sulphydryl (SH)-oxidizing reagents showed the same effects as X-rays on the ANS fluorescence. In addition, pretreatment of the membranes with SH reagents completely blocked the radiation-induced fluorescence changes. These results demonstrated that the initial cause of the radiation effect on membranes is the oxidation of membrane SH groups. There were two different steps in the development of the radiation effect on membrane structure; one is the radiation chemical reaction of SH groups, which is independent of the post-irradiation incubation temperature, and the other is markedly influenced by the temperature, particularly between 12 and 26 degrees C. Therefore it was concluded that structural disorganization of the membranes, including rearrangement of membrane components, might take place following exposure to radiation. This was supported by the fact that treatment with detergents mimicked the effect of X-irradiation. The reaction of OH and/or O2- from the aqueous environment was shown to be responsible for the membrane effect of radiation.
借助荧光探针研究了X射线照射后人红细胞膜的结构变化。发现与X射线照射（高达40 Gy）的膜结合的1-苯胺基-8-萘磺酸盐（ANS）的荧光特性（强度、偏振和离解常数）与未照射的膜有很大不同。巯基（SH）氧化试剂对ANS荧光的影响与X射线相同。此外，用SH试剂预处理膜完全阻断了辐射诱导的荧光变化。这些结果表明，辐射对膜的影响的初始原因是膜SH基团的氧化。辐射对膜结构的影响发展有两个不同阶段；一个是SH基团的辐射化学反应，它与照射后的孵育温度无关，另一个则明显受温度影响，特别是在12至26摄氏度之间。因此得出结论，膜的结构紊乱，包括膜成分的重排，可能在辐射后发生。洗涤剂处理模拟X射线照射的效果这一事实支持了这一点。已表明来自水环境的OH和/或O2-的反应是辐射对膜产生影响的原因。
