Purohit S C, Bisby R H, Cundall R B
Int J Radiat Biol Relat Stud Phys Chem Med. 1980 Aug;38(2):147-58. doi: 10.1080/09553008014551041.
Structural damage to isolated erythrocyte membranes ('ghosts') has been studied following gamma-irradiation under a variety of conditions. For this two fluorescent probes were used; one 1-anilino-8-naphthalene sulphonate probes the lipid-aqueous interface, the other, diphenylhexatriene, was used to probe the membrane fluidity. Irradiation of the membranes caused a decrease in fluorescent intensity of the added probes, and changes in polarization of fluorescence. Oxygen was found to enhance the radiation damage, and scavenger experiments showed the hydroxyl radical was the major radical species involved. The structural modifications are therefore interpreted in terms of preliminary chemical damage involving peroxidation of unsaturated lipids. In addition sensitization and protection was observed in the presence of known dose-modifying chemicals.
在多种条件下对经γ射线辐照的分离红细胞膜（“血影”）的结构损伤进行了研究。为此使用了两种荧光探针；一种是1-苯胺基-8-萘磺酸盐，用于探测脂质-水界面，另一种是二苯基己三烯，用于探测膜流动性。膜的辐照导致添加探针的荧光强度降低以及荧光偏振变化。发现氧气会增强辐射损伤，清除剂实验表明羟基自由基是主要涉及的自由基种类。因此，结构修饰被解释为涉及不饱和脂质过氧化的初步化学损伤。此外，在存在已知剂量修饰化学物质的情况下观察到了增敏和保护作用。
